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Nucleic Acids Research, 1992, Vol. 20, No. 7 1679-1684
© 1992

MOLECULAR BIOLOGY

Oligonucleotide hybridisations on glass supports: a novel linker for oligonucleotide synthesis and hybridisation properties of oligonucleotides synthesised in situ

Uwe Maskos and Edwin M. Southern

Department of Biochemistry, University of Oxford South Parks Road, Oxford OX1 3QU, UK

Received December 12, 1991. Revised March 9, 1992. Accepted March 9, 1992.

A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. These hybridisations were carried out with oligonucleotides synthesised on ‘ballotini’ solid sphere glass beads and microscope slides. The linker has a hexaethylene glycol spacer, bound to the glass via a glycidoxypropyl silane, terminating in a primary hydroxyl group that serves as starting point for automated or manual oligonucleotide synthesis.


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