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Nucleic Acids Research, 1992, Vol. 20, No. 7 1705-1710
© 1992

MOLECULAR BIOLOGY

Isolation and sequence analysis of the gene for translation elongation factor 3 from Candida albicans

Kristi K. Myers1, William A. Fonzi1 and Paul S. Sypherd*

1Department of Microbiology and Molecular Genetics, College of Medicine, University of California Irvine, CA 92717, USA

*To whom correspondence should be addressed

Received December 2, 1991. Revised March 9, 1992. Accepted March 9, 1992.

Elongation factor 3 (EF-3) is a unique and essential component of the translations! system in fungi. The gene, CEF-3, encoding elongation factor 3 has been isolated from the dimorphic fungus Candida albicans. A heterologous gene probe containing the coding region of the EF-3 gene from Saccharomyces cerevisiae (YEF-3) was used to screen three Candida albicans genomic DNA libraries. The nucleotide sequences of four partial clones were determined and combined for a full-length of 3, 671 base pairs (bp). A continuous open reading frame (ORF) of 3, 147 bp encoding a predicted protein of 1, 049 amino acids and Mr of 116, 739 dattons has been identified. A transcript of 3, 400 nucleotides is seen In Northern blot hybridization of Candida albicans total RNA using a CEF-3 gene probe. The single locus CEF-3 gene maps to chromosome 5 in the genome. Comparison of the deduced amino acid sequences of CEF-3 and YEF-3 shows 77.6%. identity. A higher degree of Identity, 86.5%, is found when comparing the carboxy-terminal portions of the two proteins. At the nucleotide level, comparison of the coding regions of the two genes exhibit 79% identity while the upstream and downstream regions show 46% and 40% identity, respectively.


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