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Nucleic Acids Research, 1992, Vol. 20, No. 8 1897-1901
© 1992

GENOME STRUCTURE AND MAPPING

Construction of a chromosome-enriched Hpall library from flow-sorted wheat chromosomes

Ming Li Wang1,2, Andrew R. Leitch2, Trude Schwarzacher2, John S. Heslop-Harrison2 and Graham Moore1

1Cambridge Laboratory, Jl Centre for Plant Science Research Colney Lane, Norwich NR4 7UJ, UK 2John Innes Institute, Jl Centre for Plant Science Research Colney Lane, Norwich NR4 7UJ, UK

Received January 27, 1992. Revised March 25, 1992. Accepted March 25, 1992.

We report here the first successful generation of a chromosome-enriched library from flow sorted plant chromosomes. Chromosomes with a characteristic DNA content (a peak) were sorted from a synchronized cell culture (TaKB1, derived from Triticum aestlvum). A Hpall library was constructed from the sorted chromosomes and half of the cloned DNA sequences analysed are unique or low copy. Approximately half of these sequences when used as probes detect sequences on wheat chromosome 4A. The generation and analysis of the chromosome library is described in detail and the prospects of using flow-sorted plant chromosomes discussed.


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