Nucleic Acids Research, 1992, Vol. 20, No. 8 1979-1981
© 1992
MOLECULAR BIOLOGY |
Alternatively spliced p53 RNA in transformed and normal cells of different tissue types
1Program of Biochemistry, Roswell Park Cancer Institute Buffalo, NY 14263, USA 2Department of Experimental Therapeutics, Grace Cancer Drug Center, Roswell Park Cancer Institute Buffalo, NY 14263, USA
*To whom correspondence should be addressed
Received December 18, 1991. Accepted March 19, 1992.
The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast cells, mouse liver and testis suggests that this alternative splicing may be universal. The level of alternatively spliced p53 RNA was increased coordinatety with that of regularly spliced p53 in 10T1/2 cells in response to epidermal growth factor. Immunoprecipitation analysis of epidermal cells using monoclonal antibodies which recognize different epitopes of p53 suggested that distinct p53 proteins may be translated from both RNA species. Considering previous observations on the potential importance of carboxyl terminal sequences in p53 function, knowledge of the ubiquitous presence of alternatively spliced p53 is important for future studies of p53 function in normal cells and in oncogenesis.
Present address: Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724-2202, USA
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