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Nucleic Acids Research, 1992, Vol. 20, No. 9 2249-2255
© 1992

MOLECULAR BIOLOGY

Tissue-specific expression in the salivary glands of transgenic mice

Thomas R. Mikkelsen, Jakob Brandt1, H.Jakob Larsen, Birte B. Larsen, Knud Poulsen2, JFErgen Ingerslev3, Nanni Din4 and J.Peter Hjorth*

Department of Molecular Biology, University of Aarhus DK-8000 Aarhus C., Denmark 1Department of Plant Biology, Royal Veterinary and Agricultural University DK-1871 Frederiksberg C., Denmark 2Department of Medical Microbiology, University of Aarhus DK-8000 Aarhus C., Denmark 3University Hospital Skejby DK-8200 Aarhus N. Denmark 4NOVO Nordisk DK-2880 Bagsvaerd, Denmark

*To whom correspondence should be addressed

Received February 11, 1992. Revised March 30, 1992. Accepted March 30, 1992.

Using a DNA construct, named Lama, derived from the murine parotid secretory protein (PSP) gene, we have obtained salivary gland specific gene expression in transgenic mice. Lama is a PSP minigene and allows analysis of the PSP gene 5' regulatory region by transgenesis. We show here that the regulatory region included in Lama with 4.6 kb of 5' flanking sequence is sufficient to direct expression specifically to the salivary glands. The expression level in the parotid gland is only about one percent of the PSP mRNA level, while that of the sublingual gland is near the PSP mRNA level. This suggests significant differences in the PSP gene regulation in the two glands. In addition, Lama is a secretory expression vector in which cDNAs or genomic fragments can be inserted. We demonstrate that the Lama construct can direct the expression of a heterologous cDNA encoding the C-terminal peptide of human factor VIII to salivary glands and that the corresponding peptide is secreted into saliva.


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