Skip Navigation

This Article
Right arrow Print PDF (6562K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (40)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Alex, R.
Right arrow Articles by Dildrop, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alex, R.
Right arrow Articles by Dildrop, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 9 2257-2263
© 1992

MOLECULAR BIOLOGY

Determination of the DNA sequence recognized by the bHLH-zip domain of the N-Myc protein

Regina Alex, Osman Sözeri, Sandra Meyer and Renate Dildrop*

Institute for Genetics, University of Cologne Weyertal 121, 5000 Cologne 41, Germany

*To whom correspondence should be addressed

Received February 10, 1992. Accepted March 31, 1992.

The DNA-binding domain of the murine N-Myc protein, comprising the basic helix-loop-helix-zipper (bHLH-zip) region was expressed as a fusion protein in E.coli. The affinity purified glutathione-S-transferase-N-Myc fusion protein (GST-N-MYC) was used to select the N-Myc specific DNA-recognition motif from a pool of random-sequence oligonucleotides. After seven rounds of binding-site selection, specifically enriched oligonucleotides were cloned and sequenced. Of 31 individual oligonucleotides whose sequences were determined, 30 contained a common DNA-motif, defining the hexameric consensus sequence CACGTG. We confirm by mutational analysis that binding of the N-Myc derived bHLH-zip domain to this motif is sequence-specific.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. A. Beierle, A. Trujillo, A. Nagaram, E. V. Kurenova, R. Finch, X. Ma, J. Vella, W. G. Cance, and V. M. Golubovskaya
N-MYC Regulates Focal Adhesion Kinase Expression in Human Neuroblastoma
J. Biol. Chem., April 27, 2007; 282(17): 12503 - 12516.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zhang, F. Li, X. Liu, L. Shen, J. Liu, J. Su, W. Zhang, Y. Deng, L. Wang, N. Liu, et al.
The Repression of Human Differentiation-related Gene NDRG2 Expression by Myc via Miz-1-dependent Interaction with the NDRG2 Core Promoter
J. Biol. Chem., December 22, 2006; 281(51): 39159 - 39168.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. S. Deeb and R. Peng
The C-514T polymorphism in the human hepatic lipase gene promoter diminishes its activity
J. Lipid Res., January 1, 2000; 41(1): 155 - 158.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H. Ding, A. M. Benotmane, G. Suske, D. Collen, and A. Belayew
Functional Interactions between Sp1 or Sp3 and the Helicase-like Transcription Factor Mediate Basal Expression from the Human Plasminogen Activator Inhibitor-1 Gene
J. Biol. Chem., July 9, 1999; 274(28): 19573 - 19580.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Feltkamp, F. F. Wiebel, S. Alberti, and J.-A. Gustafsson
Identification of a Novel DNA Binding Site for Nuclear Orphan Receptor OR1
J. Biol. Chem., April 9, 1999; 274(15): 10421 - 10429.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Lenouvel, I. Nikolaev, and B. Felenbok
In Vitro Recognition of Specific DNA Targets by AlcR, a Zinc Binuclear Cluster Activator Different from the Other Proteins of This Class
J. Biol. Chem., June 13, 1997; 272(24): 15521 - 15526.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Zhao and L.-S. Chang
The Human POLD1 Gene. IDENTIFICATION OF AN UPSTREAM ACTIVATOR SEQUENCE, ACTIVATION BY Sp1 AND Sp3, AND CELL CYCLE REGULATION
J. Biol. Chem., February 21, 1997; 272(8): 4869 - 4882.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Bachhawat, Q. Ouyang, and S. A. Henry
Functional Characterization of an Inositol-sensitive Upstream Activation Sequence in Yeast
J. Biol. Chem., October 20, 1995; 270(42): 25087 - 25095.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T Ellenberger, D Fass, M Arnaud, and S C Harrison
Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer.
Genes & Dev., April 15, 1994; 8(8): 970 - 980.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
A C Davis, M Wims, G D Spotts, S R Hann, and A Bradley
A null c-myc mutation causes lethality before 10.5 days of gestation in homozygotes and reduced fertility in heterozygous female mice.
Genes & Dev., April 1, 1993; 7(4): 671 - 682.
[Abstract] [PDF]

Home page
 DevelopmentHome page
C. Moens, B. Stanton, L. Parada, and J Rossant
Defects in heart and lung development in compound heterozygotes for two different targeted mutations at the N-myc locus
Development, January 10, 1993; 119(2): 485 - 499.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
J Charron, B A Malynn, P Fisher, V Stewart, L Jeannotte, S P Goff, E J Robertson, and F W Alt
Embryonic lethality in mice homozygous for a targeted disruption of the N-myc gene.
Genes & Dev., December 1, 1992; 6(12a): 2248 - 2257.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.