Skip Navigation

This Article
Right arrow Print PDF (2602K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (145)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yen, R.-W. C.
Right arrow Articles by Baylin, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yen, R.-W. C.
Right arrow Articles by Baylin, S. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1992, Vol. 20, No. 9 2287-2291
© 1992

MOLECULAR BIOLOGY

Isolation and characterization of the cDNA encoding human DNA methyltransferase

Ray-Whay Chiu Yen1, Paula M. Vertino1, Barry D. Nelkin1, Jane J. Yu1, Wafik El-Deiry1, Arunthathi Cumaraswamy1, Gregory G. Lennon3, Barbara J. Trask3, Paul Celano1 and Stephen B. Baylin1,2

1Oncology Center Livermore, CA 94551, USA 2Department of Medicine, The Johns Hopkins Medical Institutions Baltimore, MD 21231 Livermore, CA 94551, USA 3Human Genome Center, Biomedical Sciences Division, The Lawrence Livermore National Laboratories Livermore, CA 94551, USA

Received January 31, 1992. Revised March 30, 1992. Accepted March 30, 1992.

We have cloned a series of overlapping cDNA clones encoding a 5194 bp transcript for human DNA methyltransferase (DNA MTase). This sequence potentially codes for a protein of 1495 amino acids with a predicted molecular weight of 169 kDa. The human DNA MTase cDNA has eighty percent homology at the nucleotide level, and the predicted protein has seventy-four percent identity at the amino acid level, to the DNA MTase cDNA cloned from mouse cells. Like the murine DNA MTase, the amino terminal two-thirds of the human protein contains a cysteine-rich region suggestive of a metal-binding domain. The carboxy terminal one-third of the protein shows considerable similarity to prokaryotic (cytosine-5)-methyltransferases. The arrangement of multiple motifs conserved in the prokaryotic genes is preserved in the human DNA MTase, including the relative position of a proline cysteine dipeptide thought to be an essential catalytic site in all (cytosine-5)-methyltransferases. A single 5.2 kb transcript was detected in all human tissues tested, with the highest levels of expression observed in RNA from placenta, brain, heart and lung. DNA MTase cDNA clones were used to screen a chromosome 19 genomic cosmid library. The DNA MTase-positive cosmids which are estimated to span a genomic distance of 93 kb have been localized to 19p13.2-p13.3 by fluorescence in situ hybridization. Isolation of the cDNA for human DNA MTase will allow further study of the regulation of DNA MTase expression, and of the role of this enzyme in establishing DNA methylation patterns in both normal and neoplastic cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J BiochemHome page
I. Suetake, D. Hayata, and S. Tajima
The Amino-Terminus of Mouse DNA Methyltransferase 1 Forms an Independent Domain and Binds to DNA with the Sequence Involving PCNA Binding Motif
J. Biochem., December 1, 2006; 140(6): 763 - 776.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Levenson, T. L. Roth, F. D. Lubin, C. A. Miller, I-C. Huang, P. Desai, L. M. Malone, and J. D. Sweatt
Evidence That DNA (Cytosine-5) Methyltransferase Regulates Synaptic Plasticity in the Hippocampus
J. Biol. Chem., June 9, 2006; 281(23): 15763 - 15773.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P.-O. Esteve, D. Patnaik, H. G. Chin, J. Benner, M. A. Teitell, and S. Pradhan
Functional analysis of the N- and C-terminus of mammalian G9a histone H3 methyltransferase
Nucleic Acids Res., June 6, 2005; 33(10): 3211 - 3223.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
S. J. Lee, H.-S. Jeon, J.-S. Jang, S. H. Park, G. Y. Lee, B.-H. Lee, C. H. Kim, Y. M. Kang, W. K. Lee, S. Kam, et al.
DNMT3B polymorphisms and risk of primary lung cancer
Carcinogenesis, February 1, 2005; 26(2): 403 - 409.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P.-O. Esteve, H. G. Chin, and S. Pradhan
Human maintenance DNA (cytosine-5)-methyltransferase and p53 modulate expression of p53-repressed promoters
PNAS, January 25, 2005; 102(4): 1000 - 1005.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Flynn, J.-Y. Fang, J. A. Mikovits, and N. O. Reich
A Potent Cell-active Allosteric Inhibitor of Murine DNA Cytosine C5 Methyltransferase
J. Biol. Chem., February 28, 2003; 278(10): 8238 - 8243.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Bakker, X. Lin, and W. G. Nelson
Methyl-CpG Binding Domain Protein 2 Represses Transcription from Hypermethylated pi -Class Glutathione S-Transferase Gene Promoters in Hepatocellular Carcinoma Cells
J. Biol. Chem., June 14, 2002; 277(25): 22573 - 22580.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Majumder, K. Ghoshal, J. Datta, S. Bai, X. Dong, N. Quan, C. Plass, and S. T. Jacob
Role of de Novo DNA Methyltransferases and Methyl CpG-binding Proteins in Gene Silencing in a Rat Hepatoma
J. Biol. Chem., May 3, 2002; 277(18): 16048 - 16058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. R. Hodge, W. Xiao, P. A. Clausen, G. Heidecker, M. Szyf, and W. L. Farrar
Interleukin-6 Regulation of the Human DNA Methyltransferase (HDNMT) Gene in Human Erythroleukemia Cells
J. Biol. Chem., October 19, 2001; 276(43): 39508 - 39511.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
M. SZYF, D. J. KNOX, S. MILUTINOVIC, A. D. SLACK, and F. D. ARAUJO
How Does DNA Methyltransferase Cause Oncogenic Transformation?
Ann. N.Y. Acad. Sci., June 1, 2000; 910(1): 156 - 177.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. S. Hansen, C. Wijmenga, P. Luo, A. M. Stanek, T. K. Canfield, C. M. R. Weemaes, and S. M. Gartler
The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome
PNAS, December 7, 1999; 96(25): 14412 - 14417.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Pradhan, A. Bacolla, R. D. Wells, and R. J. Roberts
Recombinant Human DNA (Cytosine-5) Methyltransferase. I. EXPRESSION, PURIFICATION, AND COMPARISON OF DE NOVO AND MAINTENANCE METHYLATION
J. Biol. Chem., November 12, 1999; 274(46): 33002 - 33010.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-S. Hung, N. Karthikeyan, B. Huang, H.-C. Koo, J. Kiger, and C.-K. J. Shen
Drosophila proteins related to vertebrate DNA (5-cytosine) methyltransferases
PNAS, October 12, 1999; 96(21): 11940 - 11945.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D.-W. Hsu, M.-J. Lin, T.-L. Lee, S.-C. Wen, X. Chen, and C.-K. J. Shen
Two major forms of DNA (cytosine-5) methyltransferase in human somatic tissues
PNAS, August 17, 1999; 96(17): 9751 - 9756.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Singal and G. D. Ginder
DNA Methylation
Blood, June 15, 1999; 93(12): 4059 - 4070.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Bigey, J. D. Knox, S. Croteau, S. K. Bhattacharya, J. Theberge, and M. Szyf
Modified Oligonucleotides as Bona Fide Antagonists of Proteins Interacting with DNA. HAIRPIN ANTAGONISTS OF THE HUMAN DNA METHYLTRANSFERASE
J. Biol. Chem., February 19, 1999; 274(8): 4594 - 4606.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Deng and M. Szyf
Multiple Isoforms of DNA Methyltransferase Are Encoded by the Vertebrate Cytosine DNA Methyltransferase Gene
J. Biol. Chem., September 4, 1998; 273(36): 22869 - 22872.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. A. Mikovits, H. A. Young, P. Vertino, J.-P. J. Issa, P. M. Pitha, S. Turcoski-Corrales, D. D. Taub, C. L. Petrow, S. B. Baylin, and F. W. Ruscetti
Infection with Human Immunodeficiency Virus Type 1 Upregulates DNA Methyltransferase, Resulting in De Novo Methylation of the Gamma Interferon (IFN-gamma ) Promoter and Subsequent Downregulation of IFN-gamma Production
Mol. Cell. Biol., September 1, 1998; 18(9): 5166 - 5177.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
R. K. Slany, C. Lavau, and M. L. Cleary
The Oncogenic Capacity of HRX-ENL Requires the Transcriptional Transactivation Activity of ENL and the DNA Binding Motifs of HRX
Mol. Cell. Biol., January 1, 1998; 18(1): 122 - 129.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. F. Glickman, J. G. Pavlovich, and N. O. Reich
Peptide Mapping of the Murine DNA Methyltransferase Reveals a Major Phosphorylation Site and the Start of Translation
J. Biol. Chem., July 11, 1997; 272(28): 17851 - 17857.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. H. Butler, R. Slany, X. Cui, M. L. Cleary, and D. Y. Mason
The HRX Proto-oncogene Product Is Widely Expressed in Human Tissues and Localizes to Nuclear Structures
Blood, May 1, 1997; 89(9): 3361 - 3370.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. L. Tucker, D. Talbot, M. A. Lee, H. Leonhardt, and R. Jaenisch
Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene
PNAS, November 12, 1996; 93(23): 12920 - 12925.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Hollnagel, D. Schroder, and G. Gross
Domain-specific Gene Activation by Parathyroid Hormone in Osteoblastic ROS17/2.8 Cells
J. Biol. Chem., September 6, 1996; 271(36): 21870 - 21877.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. D. Araujo, S. Croteau, A. D. Slack, S. Milutinovic, P. Bigey, G. B. Price, M. Zannis-Hajopoulos, and M. Szyf
The DNMT1 Target Recognition Domain Resides in the N Terminus
J. Biol. Chem., March 2, 2001; 276(10): 6930 - 6936.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bacolla, S. Pradhan, J. E. Larson, R. J. Roberts, and R. D. Wells
Recombinant Human DNA (Cytosine-5) Methyltransferase. III. ALLOSTERIC CONTROL, REACTION ORDER, AND INFLUENCE OF PLASMID TOPOLOGY AND TRIPLET REPEAT LENGTH ON METHYLATION OF THE FRAGILE X CGG{middle dot}CCG SEQUENCE
J. Biol. Chem., May 18, 2001; 276(21): 18605 - 18613.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.