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Nucleic Acids Research, 1992, Vol. 20, No. 9 2293-2296
© 1992

MOLECULAR BIOLOGY

Swal, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5'-ATTTAAAT-3'

M. Lechner, B. Frey, F. Laue, J. Anton-Botella1, C.L. Smith1, W. Ankenbauer* and G.G. Schmitz

Boehringer Mannheim GmbH, Biochemical Research Center Nonnenwald 2, D-8122 Penzberg, Germany 1Department of Molecular and Cell Biology and Lawrence Berkeley Laboratories, Division of Chemical Biodynamics, University of California Berkeley, CA 94720, USA

*To whom correspondence should be addressed

Received January 31, 1992. Revised April 6, 1992. Accepted April 6, 1992.

A novel class-II restriction endonuclease designated Swal was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. Swal recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. Swal was used to digest chromosomal DNA from various microorganisms and human cells.


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