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Nucleic Acids Research, 1992, Vol. 20, No. 9 2313-2320
© 1992

MOLECULAR BIOLOGY

The human M creatine kinase gene enhancer contains multiple functional interacting domains

Robert V. Trask, Joseph C. Koster, Michael E. Ritchie and Joseph J. Billadello

Department of Medicine, Washington University School of Medicine St Louis, MO 63110, USA

Received January 29, 1992. Revised March 17, 1992. Accepted March 17, 1992.

Cis-elements(–933 to –641) upstream of the human M creatine kinase gene cap site contain an enhancer that confers developmental and tissue-specific expression to the chioramphenicol acetyltransferase gene in C2C12 myogenic cells transfected in culture. Division of the enhancer at –770 into a 5' fragment that includes the MyoD binding sites (–933 to –770) and a 3' fragment that includes the MEF-2 binding site (–770 to –641) resulted in two subfragments that showed minimal activity but in combination interacted in a position- and orientation-independent fashion to enhance activity of the SV4O promoter in transient transfection experiments. A 5' enhancer construct (–877 to –832) including only one (the low affinity) MyoD binding site was active when present in multiple copies. In contrast, a 3' enhancer construct (–749 to –732) including the MEF-2 binding site was inactive even when present in multiple copies. However, if the 5' construct was extended to include the high-affinity MyoD binding site (–877 to –803) the 5' and 3' constructs interacted in a position- and orientation-independent fashion to activate the SV4O promoter. Thus, the human M creatine kinase enhancer comprises multiple functional interacting domains.


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