Nucleic Acids Research, 1992, Vol. 20, No. 9 2321-2325
© 1992
MOLECULAR BIOLOGY |
Activation of the mouse DNA polymerase ß gene promoter by adenovirus type 12 E1A proteins
Laboratory of Cell Biology, Aichi Cancer Center Research Institute Chikusa-ku, Nagoya 464, Japan 1The Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108, Japan
*To whom correspondence should be addressed
Received January 17, 1992. Revised March 31, 1992. Accepted March 31, 1992.
A plasmid carrying the 5'-flanking region (–1852 to +33 with respect to the transcription initiation site) of the mouse DNA polymerase ß gene fused with the chloramphenicol acetyltransferase (CAT) gene was cotransfected into mouse N18TG2 cells with adeno virus type 12 E1 genes-expressing plasmids. Expression of E1A gene products resulted in the elevation of the CAT expression by 3 to 7 folds, but that of E1B gene product was much less effective. RNase protection analysis revealed that the activation by E1A was at the transcription process. Both the 13S E1A and the 12S E1A activated the DNA polymerase ß gene promoter, indicating that the activation domain of E1A is in a common region(s) of 13S and 12S E1A products. The major target sequence of ElA was mapped within the 10 base pair-region (–30 to –20) of the DNA poly merase ß gene promoter, which overlapped with the palindromic sequence known as the ATF(CREB)/E4F-binding consensus. The results suggest that the palindromic sequence is essential for E1A-induced transcriptional activation of the mouse DNA polymerase ß gene.