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Nucleic Acids Research, 1993, Vol. 21, No. 1 37-40
© 1993

MOLECULAR BIOLOGY

Change of the higher order structure of DNA induced by the complexation with intercalating synthetic polymer, as is visualized by fluorescence microscopy

Keiji Minagawa, Yukiko Matsuzawa, Kenichi Yoshikawa, Faculty of Engineering*, Yuichi Masubuchi1, Mitsuhiro Matsumoto1, Masao Doi1, Chitoshi Nishimura2 and Mizuo Maeda2

Graduate School of Human Informatics, Nagoya University Nagoya 464-01 1School of Engineering, Nagoya University Nagoya 464-01 2Kyushu University Fukuoka 812, Japan

*To whom correspondence should be addressed

Received October 29, 1992. Accepted December 2, 1992.

The electrophoretlc movement and Brownian motion of T4DNA, {lambda}DNA and their complexes with polyacrylamide (PAAm) via intercalative unit from 5-[(4-acryloylamino)phenyl]-3-amino-7-(dlmethylamin-o)-2-methylphenazlnium chloride were observed using fluorescence microscopy. It was found that T4DNA/PAAm complex migrates slower than T4DNA alone In gel electrophoresis, although they exhibit similar conformatlonal change during the migration. Quantitative analyses of the translatlonal diffusion of the {lambda}DNA and its complex in solution demonstrate that the DNA molecules extend due to Intercalative binding of PAAm, suggesting the pseudo-grafting structure of the complex.


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