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Nucleic Acids Research, 1993, Vol. 21, No. 14 3183-3190
© 1993

MOLECULAR BIOLOGY

The role of the preserved sequences of Dam methylase

Jean-Baptiste Guyot, Jacques Grassi1, Ulrich Hahn2,+ and Wilhelm Guschlbauer*

Service de Biochimie et Génétique Moléculaire, Bdst. Department de Biologie Cellulaire et Molénétique, Center D'Etudes de Saclay F-91191 Gif-sur-Yvette Cedex 1Service de Pharmacologie et d'lmmunologie, Département de Recherche en Imagerie, Pharmacologie et Physiologie, Centre d'Etudes de Saclay F-91191 Gif-sur-Yvette Cedex, France 2Institut für Kristallographie der Freien Universität Takustrasse 6, D-1000 Berlin 33, Germany

*To whom correspondence should be addressed

Received April 21, 1993. Revised May 17, 1993. Accepted May 17, 1993.

We have undertaken a site directed mutational analysis of two of the preserved regions in the amino acid sequence of Dam methyiase in order to characterize their role. Mutations in region IV (sequence DPPY) abolish catalytic activity and greatly affect AdoMet crosslinking. Mutants in region III display a lowered specific activity with an unchanged AdoMet crosslinking capacity. We have also made a series of deletions both at the N and C terminal parts of the protein, which have been found to provide Inactive enzyme. We discuss the significance of these results for the understanding of the functional properties of the enzyme.

+Present address: Institut für Biochemie, Medizinische Universität zu Lübeck, D-23538 Lübeck 1, Germany


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