Nucleic Acids Research, 1993, Vol. 21, No. 15 3341-3346
© 1993
MOLECULAR BIOLOGY |
A role in DNA binding for the linker sequences of the first three zinc fingers of TFIIIA
Medical Research Council, Laboratory of Molecular Biology Cambridge CB2 2QH, UK
Received June 7, 1993. Accepted June 22, 1993.
Zinc fingers of the TFIIIA type are connected by short linker sequences between the structural units. Structural investigations by 2D NMR in solution and by X-ray crystallographic analyses of complexes with DNA point to a passive role for the linkers. We have therefore investigated the influence of the linker sequence on DNA binding using as a model the first three fingers of the protein TFIIIA. Insertion of certain heterologous linkers abolishes binding, and replacement of Individual amino acids can reduce binding by factors of up to twenty-four.
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