Skip Navigation

This Article
Right arrow Print PDF (3524K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ermark, G.
Right arrow Articles by Paszkowski, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ermark, G.
Right arrow Articles by Paszkowski, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 15 3445-3450
© 1993

MOLECULAR BIOLOGY

Cytosine methylation inhibits replication of African cassava mosaic virus by two distinct mechanisms

Genadij Ermark+, Uta Paszkowski§, Markus Wohlmuth§, Orturn Mittlesten Scheid§ and Jerzy Paszkowski*

Institute of Plant Sciences, ETH-Zurich, Universitätsstrasse 2, 8092 Zurich, Swilzerland

* To whom correspondence should be addressed at: Friedrich Miescher Institute, PO Box 2543, 4002 Basel, Switzerlsnd

Received April 26, 1993. Revised June 21, 1993. Accepted June 21, 1993.

Extrachromosomally replicating viral DNA is usually free of cytosine methylation and viral templates methylated in vitro are poor substrates when used in replication assays. We have investigated the mechanism of inhibition of viral replication by DNA methylation using as a model the DNA A of African cassava mosaic virus. We have constructed two component helper systems which allow for separation of the transcriptional inhibition of viral genes necessary for replication from replication inhibition due to altered interaction between the replication complex and methylated viral DNA. Our results suggest that methylation-mediated reduction of viral replication is due to both repression mechanisms and that this provides two independent selection pressures for the maintenance of methylation-free replicons in infected cells.

+Present address: Institute of Genetics and Cytology, F. Scorina 27, 220734 Minsk, Whiterussia, USSR

§Present address: Friedrich Miescher Institute, PO Box 2543, 4002 Basel, Switzerland


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
P. Raja, B. C. Sanville, R. C. Buchmann, and D. M. Bisaro
Viral Genome Methylation as an Epigenetic Defense against Geminiviruses
J. Virol., September 15, 2008; 82(18): 8997 - 9007.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Arguello-Astorga, J. T. Ascencio-Ibanez, M. B. Dallas, B. M. Orozco, and L. Hanley-Bowdoin
High-Frequency Reversion of Geminivirus Replication Protein Mutants during Infection
J. Virol., October 15, 2007; 81(20): 11005 - 11015.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.