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Nucleic Acids Research, 1993, Vol. 21, No. 15 3473-3477
© 1993

MOLECULAR BIOLOGY

MGM101, a nuclear gene involved in maintenance of the mitochondrial genome in Saccharomyces cerevisiae

Xin-Jie Chen, Min-Xin Guan and G. Desmond Clark-Walker*

Molecular and Population Genetics Group, Research School of Biological Sciences, The Australian National University GPO Box 475, Canberra City, ACT2601, Australia

*To whom correspondence should be addressed

Received April 16, 1993. Revised June 24, 1993. Accepted June 24, 1993.

A nuclear mutation, mgm101, results in temperature sensitive loss of mitochondrial DNA (mtDNA) in the yeast Saccharomyces cerevisiae. The corresponding gene, MGM101, was isolated from a genomic DNA library by complementation. Sequence analysis shows that MGM101 encodes a positively charged protein of 269 amino acids with a calculated molecular weight of 30 kDa. This analysis also reveals that MGM101 is adjacent to the ribosomal protein gene RPS7A on chromosome X and hybridization indicates it occurs in single copy. Creation of a null mutant by targeted disruption showed that the gene has no essential cellular function, aside from its participation in mitochondrlai genome maintenance. As no counterpart has been identified in databases it is a novel protein whose role has yet to be determined. Expression of MGM101 is low on glucose medium but on galactose there is a two-fold increase in the level of the transcript.


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