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Nucleic Acids Research, 1993, Vol. 21, No. 15 3501-3505
© 1993

MOLECULAR BIOLOGY

Human snRNP polypeptide D1 promotes pre-mRNA splicing in yeast and defines nonessential yeast Smd1p sequences

Brian C. Rymond*, Luis A. Rokeach1,+ and Sallie O. Hoch1

T.H.Morgan School of Biological Sciences, University of Kentucky Lexington, KY 40506-0225, USA 1The Agouron Institute 505 Coast Boulevard South, La Jolla, CA 92037, USA

*To whom correspondence should be addressed

Received April 6, 1993. Revised June 11, 1993. Accepted June 11, 1993.

Parallel Investigations of yeast and metazoan pre-mRNA splicing have documented enormous complexity in the nucleic acid and protein components of the cellular splicing apparatus, the spliceosome. The degree to which yeast and metazoan spliceosomal proteins differ in composition and structure is currently unknown. In this report we demonstrate that the human small nuclear ribonucleoproteln (snRNP) polypeptide D1 complements the cell lethality, splicing deficiency, and snRNA instability phenotypes associated with a yeast smd1 null allele. Mutational analysis of yeast SMD1, guided by a comparison of the predicted yeast and human proteins, reveals that a large, nonconserved portion of Smd1p is dispensable for biological activity. These observations firmly establish D1 as an essential component of the cellular splicing apparatus and suggest that yeast and metazoa are remarkably similar in the polypeptides guiding early snRNP assembly.

+Present address: Department de Biochimie Faculte de Medecine, Universite de Montreal, C.P. 6128, Montreal, Quebec H3C 3J7, Canada


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