Skip Navigation

This Article
Right arrow Print PDF (8811K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pochart, P.
Right arrow Articles by Heyman, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pochart, P.
Right arrow Articles by Heyman, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 15 3513-3520
© 1993

MOLECULAR BIOLOGY

Biochemical and electron microscope analyses of the DNA reverse transcripts present in the virus-like particles of the yeast transposon Ty1. Identification of a second origin of Ty1DNA plus strand synthesis

Pascale Pochart, Bernadette Agoutin, Solange Rousset1, Roland Chanet, Vlodimierz Doroszkiewicz2 and Thérèse Heyman*

Institut Curie-Biologie, URA 1342 CNRS, Bât. 110, Centre Universitaire, 91405 Orsay Cédex, France 1Institut Curie-Biologie, URA 1292 CNRS, 26 rue d'Uim, 75231 Paris Cedex 05, France 2Institute of Microbiology, Wroclaw University Wroclaw, Poland

* To whom correspondence should be addressed

Received March 30, 1993. Revised June 14, 1993. Accepted June 14, 1993.

Transposition of Saccharomyces cerevisiae Ty1 retroelements has been shown to involve reverse transcription in intracytoplasmic virus-like particles (Ty-VLPs). Ty DNA present in the particles specified by Ty1-H3 element was found to consist of the full-length genomic DNA as well as incomplete cDNAs mainly of plus polarity. Our results indicate that identical sequences (TGGGTGGTA) are used as primers for the synthesis of plus strand cDNA, generating cDNAs of 0.345 kb (analogous to the retroviral strong-stop plus cDNA) and of 2.1 kb. Electron microscopic analyses of Ty1-VLP DNA revealed two distinct classes, one full-length and the other corresponding to 0.34 kbp molecules, the size of a LTR sequence. The full-length molecules are either completely double-stranded or only partially double-stranded at one end or at both ends. These double-stranded regions are of a length corresponding to those of incomplete plus strands detected by biochemical techniques. Double-stranded circular molecules mainly of a length corresponding to that of two-LTR circles were also detected on electron micrographs. These analyses allowed us to propose a scheme for reverse transcription in Ty particles.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
P. H. Maxwell and M. J. Curcio
Host Factors That Control Long Terminal Repeat Retrotransposons in Saccharomyces cerevisiae: Implications for Regulation of Mammalian Retroviruses
Eukaryot. Cell, July 1, 2007; 6(7): 1069 - 1080.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F.-X. Wilhelm, M. Wilhelm, and A. Gabriel
Extension and Cleavage of the Polypurine Tract Plus-strand Primer by Ty1 Reverse Transcriptase
J. Biol. Chem., November 28, 2003; 278(48): 47678 - 47684.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Sundararajan, B.-S. Lee, and D. J. Garfinkel
The Rad27 (Fen-1) Nuclease Inhibits Ty1 Mobility in Saccharomyces cerevisiae
Genetics, January 1, 2003; 163(1): 55 - 67.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. F. Lawler Jr., G. V. Merkulov, and J. D. Boeke
A Nucleocapsid Functionality Contained within the Amino Terminus of the Ty1 Protease That Is Distinct and Separable from Proteolytic Activity
J. Virol., January 1, 2002; 76(1): 346 - 354.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Wilhelm, O. Uzun, E. H. Mules, A. Gabriel, and F.-X. Wilhelm
Polypurine Tract Formation by Ty1 RNase H
J. Biol. Chem., December 7, 2001; 276(50): 47695 - 47701.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. J. Holton, T. J. D. Goodwin, M. I. Butler, and R. T. M. Poulter
An active retrotransposon in Candida albicans
Nucleic Acids Res., October 1, 2001; 29(19): 4014 - 4024.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
O. Uzun and A. Gabriel
A Ty1 Reverse Transcriptase Active-Site Aspartate Mutation Blocks Transposition but Not Polymerization
J. Virol., July 15, 2001; 75(14): 6337 - 6347.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
E. H. Mules, O. Uzun, and A. Gabriel
In Vivo Ty1 Reverse Transcription Can Generate Replication Intermediates with Untidy Ends
J. Virol., August 1, 1998; 72(8): 6490 - 6503.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. H. Mules, O. Uzun, and A. Gabriel
Replication Errors during In Vivo Ty1 Transposition Are Linked to Heterogeneous RNase H Cleavage Sites
Mol. Cell. Biol., February 1, 1998; 18(2): 1094 - 1104.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Ferbeyre, J. Bratty, H. Chen, and R. Cedergren
Cell Cycle Arrest Promotes trans-Hammerhead Ribozyme Action in Yeast
J. Biol. Chem., August 9, 1996; 271(32): 19318 - 19323.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.