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Nucleic Acids Research, 1993, Vol. 21, No. 16 3643-3646
© 1993

MOLECULAR BIOLOGY

The gene encoding DNA polymerase {alpha} from Plasmodium falciparum

JohnH. White, Brian J. Kilbey*, Erik de Vries1, Michael Goman, Pietro Alano2, Sandie Chessman, Sybil McAleese and Robert G. Ridley+

Institute of Cell and Molecular Biology, University of Edinburgh Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, UK 1Institute of Infectious Diseases and Immunology, Department of Parasitology, University of Utrecht PO Box 80165, 3508 TD Utrecht, The Netherlands 2Laboratorio Biologie Cellulare, Istituto Superiore di Sanita viale Regina Elene 299, 00161 Rome, Italy

*To whom correspondence should be addressed

Received May 28, 1993. Accepted July 12, 1993.

The gene encoding DNA polymerase {alpha} from the human malaria parasite Plasmodium falciparum has been sequenced and characterised. The deduced amlno add sequence possesses the seven sequence motifs which characterise eukaryotlc replicatlve DNA polymerases (I - VII) and four of five motifs (A - E) Identified In {alpha} DNA polymerases. The predicted protein also contains sequences which are reminiscent of Plasmodium proteins but absent from other DNA polymerases. These Include four blocks of additional amino acids interspersed with the conserved motifs of the DNA polymerases, four asparaglne rich sequences and a novel carboxy-terminal extension. Repetitive sequences similar to those found In other malarial proteins are also present. cDNA-directed PCR was used to establish the presence of these features In the {small tilde} 7kb mRNA. The coding sequence contains a single intron. The gene for DNAPol{alpha} is located on chromosome 4 and Is transcribed In both asexual and sexual erythrocytlc stages of the parasite.

+Present address: Phanna Division, Preclinical Research, F.Hoffinann-La Roche, CH4OO2 Basel, Switzerland


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