Nucleic Acids Research, 1993, Vol. 21, No. 16 3749-3754
© 1993
MOLECULAR BIOLOGY |
Subunits of the Schizosaccharomyces pombe RNA polymerase II: Enzyme purification and structure of the subunit 3 gene
1Department of Molecular Genetics, National Institute of Genetics Mishima, Shizuoka 411 2School of Life Science, Graduate University for Advanced Studies Mishima, Shizuoka 411, Japan
*To whom correspondence should be addressed
Received April 26, 1993. Revised June 28, 1993. Accepted June 28, 1993.
To improve our understanding of the structure and function of eukaryotic RNA polymerase II, we purified the enzyme from the fission yeast Schizosaccharomyces pombe. The highly purified RNA polymerase II contained more than eleven polypeptides. The sizes of the largest, the second-, and the third largest polypeptides as measured by SDS-polyacrylamide gel electrophoresis were about 210,150, and 40 kllodaltons (kDa), respectively, and are similar to those of RPB1, 2, and 3 subunits of Saccharomyces cerevislae RNA polymerase II. Using the degenerated primers designed after amino acid micro-sequencing of the 40 kDa third-largest polypeptide (subunit 3), we cloned the subunit 3 gene (rpb3) and determined its DNA sequence. Taken together with the sequence of parts of PCR-amplified cDNA, the predicted coding sequence of rpb3, interrupted by two introns, was found to encode a polypeptide of 297 amino acid residues in length with a molecular weight of 34 kDa. The S.pombe subunit 3 contains four structural domains conserved for the 
-subunit family of RNA polymerase from both eukaryotes and prokaryotes. A putative leucine zipper motif was found to exist in the C-terminal proximal conserved region (domain D). Possible functions of the conserved domains are discussed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Murata, Y. Azuma, K. Miura, Mohd. A. Rahman, M. Matsutani, M. Aoyama, H. Suzuki, K. Sugi, and M. Shirai Chlamydial SET domain protein functions as a histone methyltransferase Microbiology, February 1, 2007; 153(2): 585 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kimura, H. Suzuki, and A. Ishihama Formation of a Carboxy-Terminal Domain Phosphatase (Fcp1)/TFIIF/RNA Polymerase II (pol II) Complex in Schizosaccharomyces pombe Involves Direct Interaction between Fcp1 and the Rpb4 Subunit of pol II Mol. Cell. Biol., March 1, 2002; 22(5): 1577 - 1588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Huang and R. J. Maraia Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human Nucleic Acids Res., July 1, 2001; 29(13): 2675 - 2690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kimura and A. Ishihama Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II Nucleic Acids Res., February 15, 2000; 28(4): 952 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ishiguro, Y. Nogi, K. Hisatake, M. Muramatsu, and A. Ishihama The Rpb6 Subunit of Fission Yeast RNA Polymerase II Is a Contact Target of the Transcription Elongation Factor TFIIS Mol. Cell. Biol., February 15, 2000; 20(4): 1263 - 1270. [Abstract] [Full Text]
|
|
|
|
|
|
H. Sakurai, H. Mitsuzawa, M. Kimura, and A. Ishihama The Rpb4 Subunit of Fission Yeast Schizosaccharomyces pombe RNA Polymerase II Is Essential for Cell Viability and Similar in Structure to the Corresponding Subunits of Higher Eukaryotes Mol. Cell. Biol., November 1, 1999; 19(11): 7511 - 7518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Wlassoff, M. Kimura, and A. Ishihama Functional Organization of Two Large Subunits of the Fission Yeast Schizosaccharomyces pombe RNA Polymerase II. LOCATION OF THE CATALYTIC SITES J. Biol. Chem., February 19, 1999; 274(8): 5104 - 5113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Zhang and S. A. Darst Structure of the Escherichia coli RNA Polymerase  Subunit Amino-Terminal DomainScience, July 10, 1998; 281(5374): 262 - 266. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kimura, A. Ishiguro, and A. Ishihama RNA Polymerase II Subunits 2, 3, and 11 Form a Core Subassembly with DNA Binding Activity J. Biol. Chem., October 10, 1997; 272(41): 25851 - 25855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yao, K. Yamamoto, Y. Nishi, Y. Nogi, and M. Muramatsu Mouse RNA Polymerase I 16-kDa Subunit Able to Associate with 40-kDa Subunit Is a Homolog of Yeast AC19 Subunit of RNA Polymerases I and III J. Biol. Chem., December 20, 1996; 271(51): 32881 - 32885. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Naryshkina, D. Rogulja, L. Golub, and K. Severinov Inter- and Intrasubunit Interactions during the Formation of RNA Polymerase Assembly Intermediate J. Biol. Chem., September 29, 2000; 275(40): 31183 - 31190. [Abstract] [Full Text] [PDF]
|
|