Skip Navigation

This Article
Right arrow Print PDF (1083K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (60)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Brown, P. C.
Right arrow Articles by Silverman, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brown, P. C.
Right arrow Articles by Silverman, J. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 16 3885-3891
© 1993

MOLECULAR BIOLOGY

Cloning and regulation of the rat mdr2 gene

Paul C. Brown1,2,*, Snorri S. Thorgeirsson2 and Jeffrey A. Silverman2

1National Institute of General Medical Sciences National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA 2Laboratory of Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA

*To whom correspondence should be addressed at: National Cancer Institute, Building 37, Room 3C28, 9000 Rockville Pike, Bethesda, MD 20892-0037, USA

Received February 1, 1993. Revised June 25, 1993. Accepted June 25, 1993.

We have cloned the complete cDNA encoding the rat mdr2 gene by a combination of library screening and the polymerase chain reaction. The sequence of rat mdr2 cDNA Is highly similar to other members of the mdr gene family but the Initiation of transcription, tissue distribution and regulation of expression of rat mdr2 diverge from the other Isoforms. Primer extension analysis showed rat mdr2 mRNA to have a major transcription start point at – 277 and a minor one at approximately –518. We constructed gene specific probes for rat mdr2 and mdr1b and compared the expression patterns of these two genes. The highest expression of mdr2 mRNA was In the muscle, heart, liver and spleen. Both mdr2 and 1b mRNA levels were elevated In the livers of rats treated with CCl4 or following partial hepatectomies although the time course of induction of each gene differed. Mdr1b increased by 12 to 24 hours while mdr2 did not Increase until 48 hours. Treatment of Isolated hepatocytes or RC3 cells with cyclohexlmlde did not effect mdr2 mRNA. In contrast, mdr1b expression was increased. These data suggest that rat mdr2, unlike mdr1b, is not regulated by a negative trans-acting protein factor.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Pharmacol. Rev.Home page
L. Couture, J. A. Nash, and J. Turgeon
The ATP-Binding Cassette Transporters and Their Implication in Drug Disposition: A Special Look at the Heart.
Pharmacol. Rev., June 1, 2006; 58(2): 244 - 258.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. Yasuda, L.-b. Lan, D. Sanglard, K. Furuya, J. D. Schuetz, and E. G. Schuetz
Interaction of Cytochrome P450 3A Inhibitors with P-Glycoprotein
J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 323 - 332.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
B. R. Merriman-Smith, M. A. Young, M. D. Jacobs, J. Kistler, and P. J. Donaldson
Molecular Identification of P-Glycoprotein: A Role in Lens Circulation?
Invest. Ophthalmol. Vis. Sci., September 1, 2002; 43(9): 3008 - 3015.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
J. M. Brady, N. J. Cherrington, D. P. Hartley, S. C. Buist, N. Li, and C. D. Klaassen
Tissue Distribution and Chemical Induction of Multiple Drug Resistance Genes in Rats
Drug Metab. Dispos., July 1, 2002; 30(7): 838 - 844.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
P.H. Dixon, N. Weerasekera, K.J. Linton, O. Donaldson, J. Chambers, E. Egginton, J. Weaver, C. Nelson-Piercy, M. d. Swiet, G. Warnes, et al.
Heterozygous MDR3 missense mutation associated with intrahepatic cholestasis of pregnancy: evidence for a defect in protein trafficking
Hum. Mol. Genet., May 1, 2000; 9(8): 1209 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C. Ziemann, A. Burkle, G. F. Kahl, and K. I. Hirsch-Ernst
Reactive oxygen species participate in mdr1b mRNA and P-glycoprotein overexpression in primary rat hepatocyte cultures
Carcinogenesis, March 1, 1999; 20(3): 407 - 414.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Buchler, J. Konig, M. Brom, J. Kartenbeck, H. Spring, T. Horie, and D. Keppler
cDNA Cloning of the Hepatocyte Canalicular Isoform of the Multidrug Resistance Protein, cMrp, Reveals a Novel Conjugate Export Pump Deficient in Hyperbilirubinemic Mutant Rats
J. Biol. Chem., June 21, 1996; 271(25): 15091 - 15098.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kipp and I. M. Arias
Newly Synthesized Canalicular ABC Transporters Are Directly Targeted from the Golgi to the Hepatocyte Apical Domain in Rat Liver
J. Biol. Chem., May 19, 2000; 275(21): 15917 - 15925.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.