Skip Navigation

This Article
Right arrow Print PDF (7396K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dalyot, N.
Right arrow Articles by Oppenheim, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dalyot, N.
Right arrow Articles by Oppenheim, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 17 4031-4037
© 1993

MOLECULAR BIOLOGY

Erythropoietin triggers a burst of GATA-1 in normal human erythroid cells differentiating in tissue culture

Nava Dalyot, Eitan Fibach, Antonella Ronchi1, Eliezer A. Rachmilewitz, Sergio Ottolenghi1 and Ariella Oppenheim*

Department of Hematology, Hadassah University Hospital Jerusalem 91120, Israel 1Dipartimento di Genetica e di Biologia dei Microorganismi, Universita degli Study di Milano Via Celoria 26, Milan 20133, Italy

*To whom correspondence should be addressed

Received May 28, 1993. Revised July 19, 1993. Accepted July 19, 1993.

GATA-1 Is a central transcription-activator of erythroid differentiation. In the present work we have studied the kinetics of Its expression and activity during development of normal human erythroid progenitors, grown in primary cultures. In response to the addition of erythropoietin (Epo), the cells undergo proliferation and differentiation in a synchronized fashion. This recently developed experimental system allows biochemical dissection of erythroid differentiation in a physiological meaningful environment. No DNA-blnding activity of GATA-1 could be detected before the addition of Epo, although a very low level of mRNA was observed. Following Epo addition there was a sharp parallel rise in both mRNA and DNA-blnding activity, consistent with positive autoregulation of the GATA-1 gene. After reaching a peak on day 7–9 , both mRNA and protein activity decreased. The binding activity of the ubiquitous factor SP1 showed a biphasic pattern; Its second peak usually coincided with the GATA-1 peak, suggesting that SP1 also plays a specific role in erythroid maturation. The highest activity of GATA-1 per erythroid cell was found on day 6–8 , immediately preceding the major rise In globin gene mRNA and in the number of hemoglobinlzed cells. The results Imply that a high level of GATA-1 activity Is necessary for globin gene expression and erythroid maturation, suggesting that a requirement for a threshold concentration of GATA-1 Is part of the mechanism that determines the final steps of erythroid maturation.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 JEMHome page
C. Yu, A. B. Cantor, H. Yang, C. Browne, R. A. Wells, Y. Fujiwara, and S. H. Orkin
Targeted Deletion of a High-Affinity GATA-binding Site in the GATA-1 Promoter Leads to Selective Loss of the Eosinophil Lineage In Vivo
J. Exp. Med., June 3, 2002; 195(11): 1387 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Spadaccini, P. A. Tilbrook, M. K. Sarna, M. Crossley, J. J. Bieker, and S. P. Klinken
Transcription Factor Erythroid Kruppel-like Factor (ELKF) Is Essential for the Erythropoietin-induced Hemoglobin Production but Not for Proliferation, Viability, or Morphological Maturation
J. Biol. Chem., September 11, 1998; 273(37): 23793 - 23798.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Sato, S. Watanabe, E. Ishii, K. Tsuji, and T. Nakahata
Induction of the Erythropoietin Receptor Gene and Acquisition of Responsiveness to Erythropoietin by Stem Cell Factor in HML/SE, a Human Leukemic Cell Line
J. Biol. Chem., July 3, 1998; 273(27): 16921 - 16926.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. McDevitt, Y. Fujiwara, R. A. Shivdasani, and S. H. Orkin
An upstream, DNase I hypersensitive region of the hematopoietic-expressed transcription factor GATA-1 gene confers developmental specificity in transgenic mice
PNAS, July 22, 1997; 94(15): 7976 - 7981.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
L. H. Hoefsloot, M. P. van Amelsvoort, L. C.A.M. Broeders, D. C. van der Plas, K. van Lom, H. Hoogerbrugge, I. P. Touw, and B. Lowenberg
Erythropoietin-Induced Activation of STAT5 Is Impaired in the Myelodysplastic Syndrome
Blood, March 1, 1997; 89(5): 1690 - 1700.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K Briegel, P Bartunek, G Stengl, K. Lim, H Beug, J. Engel, and M Zenke
Regulation and function of transcription factor GATA-1 during red blood cell differentiation
Development, January 12, 1996; 122(12): 3839 - 3850.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
M J Weiss, G Keller, and S H Orkin
Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells.
Genes & Dev., May 15, 1994; 8(10): 1184 - 1197.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.