Skip Navigation

This Article
Right arrow Print PDF (2458K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (35)
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kapotas, N.
Right arrow Articles by Bellofatto, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kapotas, N.
Right arrow Articles by Bellofatto, V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 17 4067-4072
© 1993

MOLECULAR BIOLOGY

Differential response to RNA trans-splicing signals within the phosphoglycerate kinase gene cluster in Trypanosoma brucei

Nikitas Kapotas* and Vivian Bellofatto

Laboratory of Molecular Parasitology, The Rockefeller University 1230 York Avenue, New York, NY 10021, USA

*To whom correspondence should be addressed at: Box 300, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA

Received May 10, 1993. Revised July 12, 1993. Accepted July 12, 1993.

In trypanosomatlds, nuclear pre-mRNA splicing is exclusively a trans-spliclng reaction in which a capped, 39 nt exon, the mini-exon, is positioned 5' to an open reading frame. Differential RNA splicing might reflect specific mini-exon and 3' splice site Interactions. To test this hypothesis, we compared the efficiency of mini-exon addition to three natural 3' splice acceptor sites (SASs) located within a single pre-mRNA transcript. In Trypanosoma brucei, the phosphoglycerate kinase A, B and C genes (PGK A, B and C) are co-expressed as three consecutive sequences on a polycistronic pre-mRNA. This pre–mRNA gives rise to unequal amounts of PGK A, B and C mRNAs. When the SAS from each gene was placed upstream of the luciferase open reading frame and the resultant constructs transiently transfected Into T. brucei procyclic cells, luciferase activity levels indicated differential SAS utilization. Enzyme activity was low when the SAS from the A gene was present. Levels were indistinguishable when the B and C SASs were compared. After replacing luciferase with chloramphenicol acetyl transferase in the test constructs, enzyme activities were shown to directly correlate with mRNA amounts. Thus, poor splicing efficiency accounts for the differential expression of the PGK A mRNA during PGK pre-mRNA maturation. This reaction appears to reflect the polypyrimidine pattern within the 3' splice acceptor site.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. R. Haanstra, M. Stewart, V.-D. Luu, A. van Tuijl, H. V. Westerhoff, C. Clayton, and B. M. Bakker
Control and Regulation of Gene Expression: QUANTITATIVE ANALYSIS OF THE EXPRESSION OF PHOSPHOGLYCERATE KINASE IN BLOODSTREAM FORM TRYPANOSOMA BRUCEI
J. Biol. Chem., February 1, 2008; 283(5): 2495 - 2507.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. N. Siegel, K. S. W. Tan, and G. A. M. Cross
Systematic Study of Sequence Motifs for RNA trans Splicing in Trypanosoma brucei
Mol. Cell. Biol., November 1, 2005; 25(21): 9586 - 9594.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Gopal, G. A. M. Cross, and T. Gaasterland
An organism-specific method to rank predicted coding regions in Trypanosoma brucei
Nucleic Acids Res., October 15, 2003; 31(20): 5877 - 5885.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Quijada, C. Guerra-Giraldez, M. Drozdz, C. Hartmann, H. Irmer, C. Ben-Dov, M. Cristodero, M. Ding, and C. Clayton
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements
Nucleic Acids Res., October 15, 2002; 30(20): 4414 - 4424.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Zilka, S. Garlapati, E. Dahan, V. Yaolsky, and M. Shapira
Developmental Regulation of Heat Shock Protein 83 in Leishmania. 3' PROCESSING AND mRNA STABILITY CONTROL TRANSCRIPT ABUNDANCE, AND TRANSLATION IS DIRECTED BY A DETERMINANT IN THE 3'-UNTRANSLATED REGION
J. Biol. Chem., December 14, 2001; 276(51): 47922 - 47929.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. M. R. Teixeira, L. V. Kirchhoff, and J. E. Donelson
Post-transcriptional Elements Regulating Expression of mRNAs from the Amastin/Tuzin Gene Cluster of Trypanosoma cruzi
J. Biol. Chem., September 22, 1995; 270(38): 22586 - 22594.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Ramamoorthy, K. G. Swihart, J. J. McCoy, M. E. Wilson, and J. E. Donelson
Intergenic Regions between Tandem gp63 Genes Influence the Differential Expression of gp63 RNAs in Leishmania chagasi Promastigotes
J. Biol. Chem., May 19, 1995; 270(20): 12133 - 12139.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.