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Nucleic Acids Research, 1993, Vol. 21, No. 18 4344-4347
© 1993

MOLECULAR BIOLOGY

Cloning and characterization of the C.elegans histidyl-tRNA synthetase gene

Yousef G. Amaar and David L. Baillie

Institute of Molecular biology and Biochemistry, Department of Biological Sciences, Simon Fraser University Burnaby, BC V5A 1S6, Canada

Received April 19, 1993. Revised August 9, 1993. Accepted August 9, 1993.

In this paper, we report the cloning and sequencing of the C.elegans histldyl-tRNA synthetase gene. The complete genomlc sequence, and most of the cDNA sequence, of this gene Is now determined. The gene size Including flanking and coding regions Is 2230 nucleotides long. Three small Introns(45 – 50 bp long) are found to Interrupt the open reading frame. The open reading frame translates to 523 amlno acids. This ptative protein sequence shows extensive homology with the human and yeast histldyl-tRNA the histldyltRNA synthetase gene Is a single copy gene. Hence, it Is very likely that It encodes both the cytoplasmlc and the mltochondrlal histldyl-tRNA synthetases. It is likely to be trans-spliced since it contains a trans-splice site In its 5' untranslated region.


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