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Nucleic Acids Research, 1993, Vol. 21, No. 19 4444-4450
© 1993

RNA

The synthesis of oligoribonucleotides containing O6-methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage

Jane A. Grasby, P. Jonathan, G. Butler and J.Gait Michael*

MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK

To whom correspondence should be addressed

Received July 22, 1993. Accepted August 25, 1993.

The synthesis is described of oligoribonucleotides containing the modified nucleoside O6-methylguanosine. Solid-phase oligoribonucleotide assembly was carried out by use of 2'-silyl-protected nucleoside phosphoramidites, a new O6-methylguanosine-containing synthon and a mild deprotection method. The O6- methylguanosine-modified oligonucleotides were used in the study of the role of conserved residues G5, G8 and G12 in hammerhead ribozyme cleavage. Hammerheads thus substituted at any of these positions showed an approximately 75-fold reduction in kcat whereas Km was unaffected. Hammerheads with modifications at G5 or G8 showed a significant reduction in magnesium binding affinity whereas modification at G12 had no effect. The results show that the three conserved G residues play crucial but different roles in hammerhead cleavage.


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