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Nucleic Acids Research, 1993, Vol. 21, No. 19 4451-4457
© 1993

CHEMISTRY

Studies on the synthesis of nucleotidyl-peptides. II. The preparation of a nucleoidyl-peptide haVING A 5'-nucleotidyl-(p-o)-serine phosphodiester bond

Yoshihito Ueno, Ryuichi saito and Tsuijiki Hata*

Department of life Chemistry, Tokyo Institite of Technology Nagatsuta, Midoriku, Yokohama 227,Japan

*To whom correspondence should be addressed

Received July 13, 1993. Accepted August 9, 1993.

The preparation of a nucleotidyl-peptide having a thymidin-5'-yl-(P-O)-serine phosphodiester bond, [HAla-Ser(pTpT)-Phe-OH](24) is described. After condensation between the phosphorylated peptide component and an oligonucleotide component, all protecting groups could be removed under neutral conditions without ^-elimination of the pTpT from the serine moiety.


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