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Nucleic Acids Research, 1993, Vol. 21, No. 19 4524-4529
© 1993

GENOME STRUCTURE AND MAPPING

HAPPY mapping of a YAC reveals alternative haplotypes in the human immunoglobulin VH locus

Gerald Walter1,2, Ian M. Tomlinson1,2, Graham P. Cook1,2, Greg Winter1,2, Terry H. Rabbitts2 and Paul H. Dear1,2,*

1MRC Centre for Protein Engineering Hills Road, Cambridge CB2 2QH, UK 2MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK

*To whom correspondence should be addressed

Received June 18, 1993. Revised August 17, 1993. Accepted August 17, 1993.

We have identified and sequenced 14 human immunoglobulin VH segments cloned in a yeast artificial chromosome, and have used a rapid PCRbased technique (HAPPY mapping, 12) to derive the order and approximate distances between them. The sequences mapped comprise thirteen germline VH segments and one rearranged VH3 gene. Comparison of our map with other data suggests the existence of at least two distinct haplotypes, differing in the presence or absence of the consecutive genes DP-78, DP-46 and DP-64, and in the duplication of segments DP-49 and DP-65. Screening of ten individuals confirms the existence of both haplotypes, and indicates that both are common amongst the population.


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