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Nucleic Acids Research, 1993, Vol. 21, No. 19 4569-4573
© 1993

RNA

A pre-export U1 snRNP in Xenopus laevis oocyte nuclei

Michael P. Terns, Elsebet Lund and James E. Dahlberg*

Department of Biomolecular Chemistry, University of Wisconsin 1300 University Avenue, Madison, Wl 53706, USA

*To whom correspondence should be addressed

Received June 7, 1993. Revised August 11, 1993. Accepted August 11, 1993.

We demonstrate that precursors of U1 snRNA are associated with nuclear proteins prior to export to the cytoplasm. The ñ15S complexes containing pre-U1 RNA, which we call pre-export U1 snRNPs, were identified in extracts of Xenopus laevis oocyte nuclei that were synthesizing U1 RNAs from injected U1 genes. The U1 snRNP-specific A protein was associated with nuclear pre-U1 RNA since both this protein and the RNA were co-precipitated by antibodies directed against either the m7G-cap of the precursor RNA or the U1-A protein. The interaction of the U1-A protein with pre-U1 RNA required sequences in the loop II region although this region of U1 RNA was not necessary for the association of U1 A protein with mature U1 snRNPs. The U1 A protein helps protect pre-UI RNA against degradation in the nucleus.


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