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Nucleic Acids Research, 1993, Vol. 21, No. 19 4610-4614
© 1993

MOLECULAR BIOLOGY

Retroviral-type zinc fingers and glycine-rich repeats in a protein encoded by CAT2.urule;6, a Tetrahymena gene active during meiosis

Frances M. Taylor and Duane W. Martindale*

Microbiology Group, Department of Natural Resource Sciences, McGill University, Macdonald Campus, Ste Anne de Bellevue Quebec H9X 3V9, Canada

*To whom correspondence should be addressed

Received May 12, 1993. Revised August 10, 1993. Accepted August 10, 1993.

We have determined the nucleotide sequence of the cnjB gene from the ciliate Tetrahymena thermophila. This gene is transcriptionally active only during early conjugation, peaking in meiotic prophase. It contains 13 introns, four transcription start points and codes fora putative polypeptide (CnjB) of 1748 amino acids with a calculated molecular weight of 200 kilodaltons and a pi of 7.9. The coding region of cnjB has a low GC content (32% GC) and unusual codon usage. The C-terminal one-third of CnjB consists of three repetitive domains. Introns were absent in this region of cnjB. One of the repetitive domains consists of seven CCHC or retroviral-type zinc fingers, a motif found in one or two copies in retroviral nucleocapsid proteins. This motif has also been found recently in seven copies in the human nucleic-acid binding protein CNBP, in an apparent CNBP homologue in Schizosaccharomyces pombe and in one copy in a Xenopus gene active in early embryos. The other two domains are on either side of the zinc finger domain and contain a repeated glycine-rich motif seen in the heterogeneous nuclear ribonuclear proteins A1 and A22.urule;B1 as well as other proteins. Both CCHC zinc fingers and glycine-rich repeats have been found in proteins with singlestranded nucleic acid-binding activity as well as strandannealing activity. CnjB is, to our knowledge, the first protein found to contain both types of motifs.


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