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Nucleic Acids Research, 1993, Vol. 21, No. 19 4615-4620
© 1993

MOLECULAR BIOLOGY

A more efficient and specific strategy in the ablation of mRNA in Xenopus laevis using mixtures of antisense oligos

Richard Morgan, Michael Edge1 and Alan Colman*

School of Biochemistry, University of Birmingham PO Box 363, Birmingham B15 2TT 1Cancer Research Department, Zeneca Pharmaceuticals, Mereside Alderley Park, Macclesfield Cheshire SK10 4TG, UK

*To whom correspondence should be addressed at

Received May 11, 1993. Revised August 16, 1993. Accepted August 16, 1993.

Previously, antisense oligodeoxyribonucleotides (oligos) have been used to ablate specific mRNAs from the maternal RNA pool of Xenopus laevis oocytes. However, this strategy is limited by the dose of oligo which can be used and the fact that 100% cleavage of the target RNA is rare. Further, non-specific cleavage of other RNAs can also occur. We demonstrate that the use of several oligos against the histone H4 RNA results in a marked improvement in the efficiency of target degradation, due to synergistic action between oligos and the existence of RNA in at least two different secondary structures. We show, by using a set of overlapping oligos complementary to the entire H4 RNA, that the amount of oligo required for efficient target ablation is greatly lowered and non-specific effects are reduced.


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