Nucleic Acids Research, 1993, Vol. 21, No. 19 4632-4638
© 1993
MOLECULAR BIOLOGY |
Snol, a novel alternatively spliced isoform of the ski protooncogene homolog, sno
Departments of Pediatrics and Microbiology, University of Virginia Medical Center MR-4 Building Box 1131, Charlottesville, VA 22908, USA
Received January 20, 1993. Revised July 30, 1993. Accepted July 30, 1993.
We have cloned and sequenced a novel human isoform of sno, snol for insertion. Snol contains 1330 nucleotides inserted in place of 7 nucleotides of the snoN mRNA. sno is a member of the ski protooncogene family, which has been implicated in muscle development. The two previously known sno alternatively spliced isoforms are snoN (684 amino acids), and snoA (415 amino acids); snol encodes a truncated isoform of 399 amino acids (44,298 MW). Southern blot experiments show that snol contains a third alternative exon from the sno gene; a single sno gene can express all three isoforms of sno by alternative splicing. All three isoforms contain the region that is most similar to the ski proto-oncogene. The relationship between snol and snoN is analogous to that between AfosB and fosB, where a truncated form of the fosB transcription factor is produced by alternative splicing. We find conservation of human sno2.urule;-specific sequences in several mammalian species, in monkey, dog, cow, rabbit and pig, but not in rodents, whereas the common portion of the sno gene is conserved in all vertebrate species tested. SnoN, snoA, and ski mRNAs accumulate in many human tissues including skeletal muscle; the snol alternative mRNA accumulates more specifically in skeletal muscle. Snol is also expressed in rhabdomyosarcoma tumor, a tumor that contains differentiated skeletal muscle. The tissuespecific alternative splicing of human snol, an mRNA in the ski2.urule;sno gene family, and the presence of sno mRNAs in muscle are consistent with a proposed role for the sno oncogene in muscle gene regulation.
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