Skip Navigation

This Article
Right arrow Print PDF (2296K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (34)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mougel, M.
Right arrow Articles by Ehresmann, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mougel, M.
Right arrow Articles by Ehresmann, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 20 4677-4684
© 1993

RNA

Conformational analysis of the 5' leader and the gag initiation site of Mo-MuLV RNA and allosteric transitions induced by dimerization

Marylene Mougel, Naceur Tounekti1, Jean-Luc Darlix2, Jacques Paoletti1, Bernard Ehresmann and Chantal Ehresmann*

UPR 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire 15 rue Rene Descartes,67084 Strasbourg-cedex 1Unite de Biochimie, URA 147 CNRS and U140 INSERM, Institut Gustave Roussy 94805 Villejuif-cedex, France 2Labo Retro-INSERM, Ecole Normale Superieure de Lyon 46 Allee d'ltalie, 69364 Lyon-cedex, France

*To whom the correspondence should be sent

Received July 23, 1993. Revised November 3, 1993. Accepted November 3, 1993.

Dimerization of genomic RNA is a key step in the retroviral life cycle and has been postulated to be involved in the regulation of translation, encapsidation and reverse transcription. Here, we have derived a secondary structure model of nucleotides upstream from * and of the gag initiation region of Mo-MuLV RNA in monomeric and dimeric forms, using chemical probing, sequence comparison and computer prediction. The 5' domain is extensively base-paired and interactions take place between U5 and 5' leader sequences. The U5-PBS subdomain can fold in two mutually exclusive conformations: a very stable and extended helical structure (E form) in which 17 of the 18 nucleotides of the PBS are paired, or an irregular three-branch structure (B form) in which 10 nucleotides of the PBS are paired. The dimeric RNA adopts the B conformation. The monomeric RNA can switch from the E to the B conformation by a thermal treatment. If the E to B transition is associated to dimerization, it may facilitate annealing of the primer tRNAPro to the PBS by lowering the free energy required for melting the PBS. Furthermore, dimerization induces allosteric rearrangements around the SD site and the gag initiation region


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
D. Zychlinski, S. Erkelenz, V. Melhorn, C. Baum, H. Schaal, and J. Bohne
Limited complementarity between U1 snRNA and a retroviral 5' splice site permits its attenuation via RNA secondary structure
Nucleic Acids Res., October 23, 2009; (2009) gkp694v1.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. Maurel, L. Houzet, E. L. Garcia, A. Telesnitsky, and M. Mougel
Characterization of a natural heterodimer between MLV genomic RNA and the SD' retroelement generated by alternative splicing
RNA, December 1, 2007; 13(12): 2266 - 2276.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Kraunus, D. Zychlinski, T. Heise, M. Galla, J. Bohne, and C. Baum
Murine Leukemia Virus Regulates Alternative Splicing through Sequences Upstream of the 5{middle dot} Splice Site
J. Biol. Chem., December 8, 2006; 281(49): 37381 - 37390.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Kanevsky, N. Vasilenko, H. Dumay-Odelot, and P. Fosse
In vitro characterization of a base pairing interaction between the primer binding site and the minimal packaging signal of avian leukosis virus genomic RNA
Nucleic Acids Res., December 15, 2003; 31(24): 7070 - 7082.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C. Deffaud and J.-L. Darlix
Characterization of an Internal Ribosomal Entry Segment in the 5' Leader of Murine Leukemia Virus env RNA
J. Virol., January 1, 2000; 74(2): 846 - 850.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
A. H. Lund, J. G. Mikkelsen, J. Schmidt, M. Duch, and F. S. Pedersen
The Kissing-Loop Motif Is a Preferred Site of 5' Leader Recombination during Replication of SL3-3 Murine Leukemia Viruses in Mice
J. Virol., November 1, 1999; 73(11): 9614 - 9618.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. G. Mikkelsen, A. H. Lund, M. Duch, and F. S. Pedersen
Recombination in the 5' Leader of Murine Leukemia Virus Is Accurate and Influenced by Sequence Identity with a Strong Bias toward the Kissing-Loop Dimerization Region
J. Virol., September 1, 1998; 72(9): 6967 - 6978.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.ép. Vagner, A. Waysbort, M. Marenda, M.-C. Gensac, F.ço. Amalric, and A.-C. Prats
Alternative Translation Initiation of the Moloney Murine Leukemia Virus mRNA Controlled by Internal Ribosome Entry Involving the p57/PTB Splicing Factor
J. Biol. Chem., September 1, 1995; 270(35): 20376 - 20383.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.