Skip Navigation

This Article
Right arrow Print PDF (3308K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nashimoto, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nashimoto, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 20 4696-4702
© 1993

RNA

3' truncated tRNAArg is essential for in vitro specific cleavage of partially synthesized mouse 18S rRNA

Masayuki Nashimoto*

Department of Biochemistry, Hokkaido University School of Medicine Sapporo 060, Japan

Received July 15, 1993. Revised August 31, 1993. Accepted August 31, 1993.

An extensive analysis of oligonucleotide interactions was carried out by hybridising a synthetic pool of 256 10mers, A(C,T)gA, representing all oligopyrimidine octamer sequences to an array of four copies of all 256 different octapurine sequences. The resulting 256 duplexes were quantified by phosphorimaging and analysed to determine the dependence of duplex formation on base composition, sequence, and salt concentration. The results show that the base composition dependence of duplex formation can be reduced by high concentrations of tetramethylammonium chloride. This chaotropic solvent also increases duplex yield by up to fiftyfold.

*Present address: Life Science Research Laboratory, Japan Tobacco Inc.,


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
H. S. Shibata, H. Takaku, M. Takagi, and M. Nashimoto
The T Loop Structure Is Dispensable for Substrate Recognition by tRNase ZL
J. Biol. Chem., June 10, 2005; 280(23): 22326 - 22334.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Takaku, A. Minagawa, M. Takagi, and M. Nashimoto
The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity
Nucleic Acids Res., August 18, 2004; 32(15): 4429 - 4438.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Takaku, A. Minagawa, M. Takagi, and M. Nashimoto
A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules
Nucleic Acids Res., June 28, 2004; 32(11): e91 - e91.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Takaku, A. Minagawa, M. Takagi, and M. Nashimoto
A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease
Nucleic Acids Res., May 1, 2003; 31(9): 2272 - 2278.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.