Nucleic Acids Research, 1993, Vol. 21, No. 20 4721-4725
© 1993
MOLECULAR BIOLOGY |
Coordinate translational regulation in the syntheses of elongation factor 1
and ribosomal proteins in Xenopus laevis
Dipartimento di Biologia, Universita di Roma Tor Vergata', via della Ricerca Scientifica, 00133 Rome, Italy
*To whom the correspondence should be addressed
Received July 15, 1993. Revised September 7, 1993. Accepted September 7, 1993.
The regulation of the synthesis of elongation factor 1a (EF-1a) in Xenopus laevis has been analyzed from the point of view of translational control. The 5' end of EF-1a mRNA, examined by primer extension, revealed the presence of a terminal pyrimidine tract that is characteristic of ribosomal protein mRNAs (rp-mRNAs). We have then compared the translation pattern of EF-1a and rp-mRNAs during Xenopus embryogenesis and in Xenopus cultured cells during growth rate changes. In Xenopus embryos EF-1a transcripts, that appear after midblastula transition, are initially mostly localized on mRNP and translationally inactive. Only later in embryogenesis, together with rp-mRNAs, they are gradually recruited on polysomes. Also in Xenopus cells B 3.2, EF-1a mRNA shows a distribution change similar to an rp-mRNA: part of it moves from polysomesto mRNP during serum deprivation and goes back on polysomes after restitution of serum to the culture. Moreover EF-1a mRNA, similarly to rp-mRNAs, is always localized on mRNP or fully loaded on polysomes but never on small polysomes. Therefore EF-1a mRNA for structural features and translation behavior can be included in the 'regulatory' group of rp-mRNAs.
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