Nucleic Acids Research, 1993, Vol. 21, No. 20 4726-4733
© 1993
MOLECULAR BIOLOGY |
Cpf1 protein induced bending of yeast centromere DNA element I
Institut für Mikro- und Molekularbiologie, Justus Liebig Universität Gießen Frankfurter Straße 107, 35392 Gießen, Germany
*To whom the correspondence should be addressed
Received July 15, 1993. Revised September 9, 1993. Accepted September 9, 1993.
The centromere complex is a muiticomponent structure essential for faithful chromosome transmission. Here we show that the S.cerevisiae centromere protein Cpf 1 bends centromere DNA element I (CDEI) with the bend angle ranging from 66° to 71°. CDEI DNA sequences that carry point mutations which lead to reduced Cpf 1 binding affinity and in vivo centromere activity are still able to show bending. The Cpf1 induced bend is directed towards the major groove with the bend centre located in CDEI. An intrinsic bend cannot replace the Cpf1 induced DNA bend for in vivo centromere function. An in vivo phasing experiment suggests that both the distance and the correct spatial arrangement of the CDEI/Cpf1 complex to CDEII and CDEIII are important for optimal centromere function.
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