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Nucleic Acids Research, 1993, Vol. 21, No. 20 4734-4738
© 1993

MOLECULAR BIOLOGY

Nucleosomal structure at hyperacetylated loci probed in nuclei by DNA-histone crosslinking

Konstantin K. Ebralidse+, Tim R. Hebbes, Alison L. Clayton, Alan W. Thorne and Colyn Crane-Robinson*

Biophysics Laboratories, University of Portsmouth St Michael's Building, White Swan Road, Portsmouth P01 2DT, UK

*To whom correspondence should be addressed.

Received July 12, 1993. Revised August 27, 1993. Accepted August 27, 1993.

Chemically induced histone-DNA crosslinking in nuclei is used to monitor structural changes in chromosomal domains containing hyperacetylated histones. Core particles harbouring the crosslinks are immunofractionated with antibodies specific for acetylated histones. Crosslinking is revealed by gel separation of tryptic peptides from core histones that carry 32Plabelled residual nucleotide. The large number of DNAhistone crosslinks retained indicates that acetylated core histone tails are not totally displaced from the DNA. Changes in the patterns of crosslinked peptides imply a restructuring of hyperacetylated histone-DNA interactions at several points within the nucleosome. This demonstrates that a distinct conformational state is adopted in acetylated nucleosomes, known to be concentrated at transcriptionally active loci.

+Present address: Harvard University, Department of Biochemistry and Molecular Biology, 7 Divinity Avenue, Cambridge, MA 02138-2092, USA and Permanent address: W. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., Moscow 117984, Russia.


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