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Nucleic Acids Research, 1993, Vol. 21, No. 20 4762-4768
© 1993

RNA

Cis-elements involved in alternative splicing in the rat ß-tropomyosin gene: the 3'-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells

Wei Guo1,2 and David M. Helfman1,*

1Cold Spring Harbor Laboratory PO Box 100, Cold Spring Harbor, NY 11724 2Department of Biochemistry and Cell Biology, State University of New York Stony Brook, NY 11794, USA

*To whom correspondence should be addressed

Received July 6, 1993. Revised August 24, 1993. Accepted August 24, 1993.

We have been using the rat ß-tropomyosin ((3-TM) gene as a model system to study the mechanism of alternative splicing. The ß-TM gene spans 10 kb with 11 exons and encodes two distinct isoforms, namely skeletal muscle ß-TM and f ibroblast TM-1. Exons 1-5, 8, and 9 are common to all mRNAs expressed from this gene. Exons 6 and 11 are used in fibroblasts, as well as in smooth muscle cells, whereas exons 7 and 10 are used exclusively in skeletal muscle cells. Our previous studies localized the critical elements for regulated alternative splicing to sequences within exon 7 and the adjacent upstream intron. We also demonstrated that these sequences function, in part, to regulate splicesite selection in vivo by interacting with cellular factors that block the use of the skeletal muscle exon in nonmuscle cells (1). Here we have further characterized the critical c/s-acting elements involved in alternative splice site selection. Our data demonstrate that exon 7 and its flanking intron sequences are sufficient to regulate the suppression of exon 7 in nonmuscle cells when flanked by heterologous exons derived from adenovirus. We have also shown by both in vivo and in vitro assays that the blockage of exon 7 in nonmuscle cells is primarily at its 3'-splice site. A model is presented for regulated alternative splicing in both skeletal muscle and nonmuscle cells.


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