Skip Navigation

This Article
Right arrow Print PDF (2167K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (85)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Huang, H.
Right arrow Articles by Ma, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, H.
Right arrow Articles by Ma, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 20 4769-4776
© 1993

MOLECULAR BIOLOGY

Isolation and characterization of the binding sequences for the product of the Arabidopsis floral homeotic gene AGAMOUS

Hai Huang, Yukiko Mizukami, Yi Hu and Hong Ma*

Cold Spring Harbor Laboratory Cold Spring Harbor, NY 11724-2212, USA

*To whom correspondence should be addressed

Received July 2, 1993. Revised August 30, 1993. Accepted August 30, 1993.

The Arabidopsis floral homeotic geneAGAMOUS (AG) is required for normal flower development. The deduced AG protein contains a region which shares substantial sequence similarity with the DNA-binding domains of known transcription factors, SRF (human) and MCM1 (yeast). Therefore, it is likely that AG is also a DNA-binding protein regulating transcription of floral genes. We describe here several experiments to characterize AG-DNA binding in vitro. We show that AG indeed binds a DNA sequence matching the consensus of SRF targets. Further, we have selected the AGbinding sequences from a pool of random oligonucleotides, and deduced an AG-binding consensus sequence of TT(A/T)CC(A/T)(A/t)2(T/A)NNGG(-G)(A/t)2. We have demonstrated that AG binds to the consensus region of three of the oligonucleotides by footprinting analysis. Finally, we have examined AG's relative binding affinity for different sequences, as compared to SRF, by gel mobility shift analysis. Our results indicate that AG is a sequence-specific DNA-binding protein, and that the AG-binding consensus sequence is similar to those of MCM1 and SRF.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
Y. Zheng, N. Ren, H. Wang, A. J. Stromberg, and S. E. Perry
Global Identification of Targets of the Arabidopsis MADS Domain Protein AGAMOUS-Like15
PLANT CELL, September 1, 2009; 21(9): 2563 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
F. Ming and H. Ma
A terminator of floral stem cells
Genes & Dev., August 1, 2009; 23(15): 1705 - 1708.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
B. Sun, Y. Xu, K.-H. Ng, and T. Ito
A timing mechanism for stem cell maintenance and differentiation in the Arabidopsis floral meristem
Genes & Dev., August 1, 2009; 23(15): 1791 - 1804.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. Melzer, W. Verelst, and G. Theissen
The class E floral homeotic protein SEPALLATA3 is sufficient to loop DNA in 'floral quartet'-like complexes in vitro
Nucleic Acids Res., January 1, 2009; 37(1): 144 - 157.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
W.-C. Tsai, Z.-J. Pan, Y.-Y. Hsiao, M.-F. Jeng, T.-F. Wu, W.-H. Chen, and H.-H. Chen
Interactions of B-class complex proteins involved in tepal development in Phalaenopsis orchid
Plant Cell Physiol., May 1, 2008; 49(5): 814 - 824.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Ito, K.-H. Ng, T.-S. Lim, H. Yu, and E. M. Meyerowitz
The Homeotic Protein AGAMOUS Controls Late Stamen Development by Regulating a Jasmonate Biosynthetic Gene in Arabidopsis
PLANT CELL, November 1, 2007; 19(11): 3516 - 3529.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. A. Eckardt
Elucidating the Function of Synergid Cells: A Regulatory Role for MYB98
PLANT CELL, August 1, 2007; 19(8): 2320 - 2321.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. O. Steffens, C. Galuschka, M. Schindler, L. Bulow, and R. Hehl
AthaMap web tools for database-assisted identification of combinatorial cis-regulatory elements and the display of highly conserved transcription factor binding sites in Arabidopsis thaliana
Nucleic Acids Res., July 1, 2005; 33(suppl_2): W397 - W402.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. A. Benedito, P. B. Visser, J. M. van Tuyl, G. C. Angenent, S. C. de Vries, and F. A. Krens
Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis
J. Exp. Bot., June 1, 2004; 55(401): 1391 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Wellmer, J. L. Riechmann, M. Alves-Ferreira, and E. M. Meyerowitz
Genome-Wide Analysis of Spatial Gene Expression in Arabidopsis Flowers
PLANT CELL, May 1, 2004; 16(5): 1314 - 1326.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T.-Y. Tzeng, H.-C. Liu, and C.-H. Yang
The C-terminal Sequence of LMADS1 Is Essential for the Formation of Homodimers for B Function Proteins
J. Biol. Chem., March 12, 2004; 279(11): 10747 - 10755.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. O. Steffens, C. Galuschka, M. Schindler, L. Bulow, and R. Hehl
AthaMap: an online resource for in silico transcription factor binding sites in the Arabidopsis thaliana genome
Nucleic Acids Res., January 1, 2004; 32(90001): D368 - 372.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Tang and S. E. Perry
Binding Site Selection for the Plant MADS Domain Protein AGL15: AN IN VITRO AND IN VIVO STUDY
J. Biol. Chem., July 18, 2003; 278(30): 28154 - 28159.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
R. L. Hong, L. Hamaguchi, M. A. Busch, and D. Weigel
Regulatory Elements of the Floral Homeotic Gene AGAMOUS Identified by Phylogenetic Footprinting and Shadowing
PLANT CELL, June 1, 2003; 15(6): 1296 - 1309.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
T.-Y. Tzeng, H.-Y. Chen, and C.-H. Yang
Ectopic Expression of Carpel-Specific MADS Box Genes from Lily and Lisianthus Causes Similar Homeotic Conversion of Sepal and Petal in Arabidopsis
Plant Physiology, December 1, 2002; 130(4): 1827 - 1836.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Gollop, S. Even, V. Colova-Tsolova, and A. Perl
Expression of the grape dihydroflavonol reductase gene and analysis of its promoter region
J. Exp. Bot., June 1, 2002; 53(373): 1397 - 1409.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. G. Franks, C. Wang, J. Z. Levin, and Z. Liu
SEUSS, a member of a novel family of plant regulatory proteins, represses floral homeotic gene expression with LEUNIG
Development, January 1, 2002; 129(1): 253 - 263.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
M. A. Koch, B. Weisshaar, J. Kroymann, B. Haubold, and T. Mitchell-Olds
Comparative Genomics and Regulatory Evolution: Conservation and Function of the Chs and Apetala3 Promoters
Mol. Biol. Evol., October 1, 2001; 18(10): 1882 - 1891.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. K. Deyholos and L. E. Sieburth
Separable Whorl-Specific Expression and Negative Regulation by Enhancer Elements within the AGAMOUS Second Intron
PLANT CELL, October 1, 2000; 12(10): 1799 - 1810.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
J. L. Riechmann, T. Ito, and E. M. Meyerowitz
Non-AUG Initiation of AGAMOUS mRNA Translation in Arabidopsis thaliana
Mol. Cell. Biol., December 1, 1999; 19(12): 8505 - 8512.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y.-H. Moon, H.-G. Kang, J.-Y. Jung, J.-S. Jeon, S.-K. Sung, and G. An
Determination of the Motif Responsible for Interaction between the Rice APETALA1/AGAMOUS-LIKE9 Family Proteins Using a Yeast Two-Hybrid System
Plant Physiology, August 1, 1999; 120(4): 1193 - 1204.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
S. R. Lockhart, M. Nguyen, T. Srikantha, and D. R. Soll
A MADS Box Protein Consensus Binding Site Is Necessary and Sufficient for Activation of the Opaque-Phase-Specific Gene OP4 of Candida albicans
J. Bacteriol., December 15, 1998; 180(24): 6607 - 6616.
[Abstract] [Full Text]

Home page
 Plant CellHome page
M. M. Kater, L. Colombo, J. Franken, M. Busscher, S. Masiero, M. M. Van Lookeren Campagne, and G. C. Angenent
Multiple AGAMOUS Homologs from Cucumber and Petunia Differ in Their Ability to Induce Reproductive Organ Fate
PLANT CELL, February 1, 1998; 10(2): 171 - 182.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
L. Sieburth, G. Drews, and E. Meyerowitz
Non-autonomy of AGAMOUS function in flower development: use of a Cre/loxP method for mosaic analysis in Arabidopsis
Development, January 11, 1998; 125(21): 4303 - 4312.
[Abstract] [PDF]

Home page
 DevelopmentHome page
J. Tilly, D. Allen, and T Jack
The CArG boxes in the promoter of the Arabidopsis floral organ identity gene APETALA3 mediate diverse regulatory effects
Development, January 5, 1998; 125(9): 1647 - 1657.
[Abstract] [PDF]

Home page
 DevelopmentHome page
T. Hill, C. Day, S. Zondlo, A. Thackeray, and V. Irish
Discrete spatial and temporal cis-acting elements regulate transcription of the Arabidopsis floral homeotic gene APETALA3
Development, January 5, 1998; 125(9): 1711 - 1721.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
B McGonigle, K Bouhidel, and V F Irish
Nuclear localization of the Arabidopsis APETALA3 and PISTILLATA homeotic gene products depends on their simultaneous expression.
Genes & Dev., July 15, 1996; 10(14): 1812 - 1821.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
H Ma
The unfolding drama of flower development: recent results from genetic and molecular analyses.
Genes & Dev., April 1, 1994; 8(7): 745 - 756.
[PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.