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Nucleic Acids Research, 1993, Vol. 21, No. 21 4873-4878
© 1993

MOLECULAR BIOLOGY

The Major Histocompatability Complex (MHC)Ea promoter: sequences and factors at the initiation site

R. Mantovani, L. Tora, V. Moncollin, J.M. Egly, C. Benoist* and D. Mathis

Laboratoire de Géneétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire de Génie Génétique de I'INSERM, Institut de Chimie Biologique, Faculté de Médecine 11 rue Humann, 67085 Strasbourg Cedex, France

*To whom correspondence should be addressed at: LGME, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France

Received August 10, 1993. Revised September 16, 1993. Accepted September 16, 1993.

We have analysed the function of sequences in the TATA/initiator region of the promoter of Ea, a class II gene of the Major Histocompatibility Complex. We find that the Ea promoter contains an initiator element with a strong influence on transcription. We also find that the Ea promoter does contain a bona fide TATA box, which can be recognized by the TATA binding protein (TBP), and that TBP is required for transcriptional activity. For activity, TBP must be included within a larger TFIID complex, as Ea transcription in a heattreated extract can be restored by immunopurified TFIID but not by TBP alone. On the other hand, the TATA motif can be eliminated without significantly affecting either the efficiency or the startsites of transcription. This suggests that TBP, even in this TATA-containing promoter, is held in place by other components of the initiation complex, regardless of its affinity for the underlying DNA.


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