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Nucleic Acids Research, 1993, Vol. 21, No. 21 4967-4974
© 1993

MOLECULAR BIOLOGY

Codon reading scheme in Mycoplasma pneumoniae revealed by the analysis of the complete set of tRNA genes

Philippe Simoneau1,+, Cheng-ming Li1, Steve Loechel1, Rainer Wenze4, Richard Herrmann4 and Ping-chuan Hu1,2,3,*

1Departments of Pediatrics, University of North Carolina Chapel Hill, NC 27599, USA 2Departments of Microbiology and Immunology University of North Carolina Chapel Hill, NC 27599, USA 3Center of Environmental Medicine, University of North Carolina Chapel Hill, NC 27599, USA 4department of Microbiology, University of Heidelberg Im Neuenheimer Feld 282, D-6900, Heidelberg, Germany

*To whom correspondance should be addressed

Received May 5, 1993. Revised September 17, 1993. Accepted September 17, 1993.

The 33 genes encoding the complete set of tRNA species in Mycoplasma pneumoniae have been cloned and sequenced. They are organized into 5 clusters in addition to 9 single genes. No redundant gene was found, indicating that 33 tRNAs correspond to 32 different anticodons and decode all 62 codons used in this organism. There is only one single tRNA for each of theAla, Leu, Pro, and Val family boxes. Therefore, a simplified decoding system resembling that recently described for Mycoplasma capricolum (1) has to also exist in M.pneumoniae. However, analysis of the anticodon set and codon usage revealed features characteristic of the latter: (i) there is no obvious preference toward AT rich synonymous codons, (ii) CGG codons are assigned for arginine and are translated by tRNA Arg(UCG), and (iii) CNN or GNN anticodons are encountered in the Ser, Thr, Arg, and Gly family boxes. We thus propose that this codonanticodon recognition pattern has emerged in the 'M.pneumoniae cluster' under a genomic economization strategy but without the influence of AT pressure.

+Present address: Faculty des Sciences, Universite d'Angers, 2 Boulevard Lavoisier, 49045 Angers Cedex, France


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