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Nucleic Acids Research, 1993, Vol. 21, No. 23 5289-5293
© 1993

SURVEY AND SUMMARY

The P1 family: a new class of nuclear mammalian proteins related to the yeast Mem replication proteins

Bing Hu, Roland Burkhart, Dorothea Schulte, Christine Musahl and Rolf Knippers*

Division of Biology, Universiteèt Konstanz D 78434 Konstanz, Germany

*To whom correspondence should be addressed

Received September 3, 1993. Revised October 19, 1993. Accepted October 19, 1993.

Monospecific antibodies against an oligopeptide, conserved among the Mem class of yeast replication proteins, were used to screen a human cDNA library. Eight of the isolated cDNA clones have the potential to code for sections of proteins with high sequence similarities to the yeast proteins Mcm3 and Cdc46 from Saccharomyces cerevisiae and Cdc21 from S. pombe. Our results establish a novel and highly conserved family of nuclear proteins in mammalian cells.


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