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Nucleic Acids Research, 1993, Vol. 21, No. 23 5345-5350
© 1993

GENOME STRUCTURE AND MAPPING

Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II

Joeül Acker, Marguerite Wintzerith, Marc Vigheron* and Claude Kedinger

Laboratoire de Geéneétique Moleéculaire des Eucaryotes (CNRS), Uniteé 184 de Biologie Moleéculaire et de Geénie Geéneétique (INSERM), Institut de Chimie Biologique 11 rue Humann, 67085 Strasbourg Cedex, France

*To whom correspondence should be addressed

Received September 3, 1993. Revised October 19, 1993. Accepted October 19, 1993.

The structure of the gene encoding the 14.5 kDa subunit of the human RNA polymerase II (or B) has been elucidated. The gene consists of six exons, ranging from 52 to over 101 bp, interspaced with five introns ranging from 84 to 246 bp. It is transcribed into three major RNA species, present at low abundance in exponentially growing HeLa cells. The corresponding messenger RNAs contain the same open reading frame encoding a 125 amino acid residue protein, with a calculated molecular weight of 14,523 Da. This protein (named hRPB14.5) shares strong homologies with the homologous polymerase subunits encoded by the Drosophila (Rpll15) and yeast (RPB9) genes. Cysteines characteristic of two zinc fingers are conserved in all three corresponding sequences and, like the yeast protein, the hRPB14.5 subunit exhibits zinc-binding activity.


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