Nucleic Acids Research, 1993, Vol. 21, No. 23 5372-5376
© 1993
MOLECULAR BIOLOGY |
Distinct DNA binding preferences for the c-Myc/Max and Max/Max dimers
Growth Control and Development Laboratory, Imperial Cancer Research Fund PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
*To Whom correspond should be addressed
Received August 27, 1993. Revised October 6, 1993. Accepted October 6, 1993.
The transcription factor c-Myc and its dimerisation partner Max are members of the basic/helix-loophelix/leucine-zipper (bHLH-Z) family and bind to the DNA core sequence CACGTG. Using a site-selection protocol, we determined the complete 12 base pair consensus binding sites of c-Myc/Max (RACCACGTGGTY) and Max/Max (RANCACGTGNTY) dimers. We find that the c-Myc/Max dimer fails to bind the core when it is flanked by a 5'T or a 3'A, while the Max/Max dimer readily binds such sequences. Furthermore we show that inappropriate flanking sequences preclude transactivation by c-Myc in vivo. In conclusion, Max/Max dimers are less discriminatory than c-Myc/Max and may regulate other genes in addition to c-Myc/Max targets.
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