Skip Navigation

This Article
Right arrow Print PDF (1210K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (55)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by DOlinnaya, N. G.
Right arrow Articles by Shabarova, Z. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by DOlinnaya, N. G.
Right arrow Articles by Shabarova, Z. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 23 5403-5407
© 1993

CHEMISTRY

Oligonucleotide circularization by template-directed chemical ligation

Nina G. DOlinnaya1, Marta Blumenfeld*, Irena N. Merenkova1, Tatiana S. Oretskaya1, Natalia Krynetskaya1, Marina G. Ivanovskaya1, Marc Vasseur1 and Zoe A. Shabarova1

GENSET, 1 Passage Etienne Delaunay 75011 Paris, France 1Joint Laboratory GENSET-Laboratory of Chemistry of Nucleic Acid Moscow State University, Moscow 119899, Russia

*To whom correspondence should be addressed

Received August 19, 1993. Revised October 26, 1993. Accepted October 26, 1993.

An efficient method for producing the covalent closure of oligonucleotides on complementary templates by the action of BrCN was developed. A rational design of linear precursor oligonucleotides was studied, and the effect of factors such as oligonucleotide concentration and oligomer-template length ratio was evaluated. The efficiency of circularization was shown to correlate well with the secondary structure of the precursor oligomer (as predicted by a simple computer analysis), hairpinlike structures bearing free termini clearly favouring the circularization reaction. A novel idea, consisting of the incorporation of non-nucleotide insertions in the precursor oligomer (namely, 1,2-dideoxy-D-ribofuranose residues), may render this method universal and highly effective. An original set of assays was developed to confirm the circular structure of the covalently closed oligonucleotides.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
Y. WANG and S. K. SILVERMAN
A general two-step strategy to synthesize lariat RNAs
RNA, February 1, 2006; 12(2): 313 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Qi and R. H. Shafer
Covalent ligation studies on the human telomere quadruplex
Nucleic Acids Res., June 2, 2005; 33(10): 3185 - 3192.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Zhou, G. Chen, Y. Wang, Q. Zhang, M. Yang, and T. Li
Synthesis of unimolecularly circular G-quadruplexes as prospective molecular probes
Nucleic Acids Res., December 8, 2004; 32(21): e173 - e173.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M Nilsson, H Malmgren, M Samiotaki, M Kwiatkowski, B. Chowdhary, and U Landegren
Padlock probes: circularizing oligonucleotides for localized DNA detection
Science, September 30, 1994; 265(5181): 2085 - 2088.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.