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Nucleic Acids Research, 1993, Vol. 21, No. 24 5583-5588
© 1993

RNA

Editing does not exist for mammalian selenocysteine tRNAs

Ruüdiger Amberg, Carsten Urban, Birgit Reuner+, Peter Scharff, Steven C. Pomerantz1, James A. McCloskey1 and Hans J. Gross*

Institut fuür Biochemie, Biozentrum, Am Hubland D-97074 Wuürzburg, Germany 1Department of Medicinal Chemistry, University of Utah Salt Lake City, UT 84112, USA

*To whom correspondence should be addressed

Received September 21, 1993. Revised November 1, 1993. Accepted November 1, 1993.

It has been reported that selenocysteine tRNA from bovine liver is completely edited to two isoacceptor species, called tRNA(ser)secNCA and tRNA(ser)secCmCA, which differ from the gene sequence. We used direct tRNA sequencing, mobility shift analyses, primer extension, restriction enzyme digestion and single strand conformational polymorphism (SSCP) analyses of products from reverse transcription coupled with polymerase chain reaction (RT&2.urule;PCR), sequencing of RT&2.urule;PCR products and HPLC-coupled mass spectrometry to reproduce this result and show here that editing of these tRNAs does not occur.

+Present address: Max-Planck-Institut fur Biochemie, D-82152 Martinsried, Germany


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