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Nucleic Acids Research, 1993, Vol. 21, No. 24 5782-5785
© 1993

METHODS

PCR primed with VNTR core sequences yields species specific patterns and hypervariable probes

Daniel D. Heath+, George K. Lwama1 and Robert H. Devlin*

Fisheries and Oceans Canada, West Vancouver Laboratory 4160 Marine Drive, West Vancouver, BC V7V 1N6 1Department of Animal Science, and Canadian Bacterial Disease Network, University of British Columbia 2357 Main Mall, Suite 248, Vancouver, BC V6T 1Z4, Canada

*To whom correspondence should be addressed

Received June 24, 1993. Revised November 1, 1993. Accepted November 1, 1993.

The use of genomic DNA-based techniques in ecological and evolutionary studies has been limited by the availability of suitable probes for species of interest due to the technical difficulty of isolating and applying such probes. We have developed a simple technique that directs polymerase chain reaction (PCR) amplification to regions rich in variable number of tandem repeats (VNTRs). By using published VNTR core sequences as primers in PCRs, fragments were amplified that showed little variation within a species, but did show differences between species. When the amplified fragments were used as probes with genomic DNA Southern blots they produced hypervariable singlelocus or few-locus patterns in fish, birds, and humans. We have named this procedure as Directed Amplification of Minisatellite-region DNA (DAMD).

+Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA


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