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Nucleic Acids Research, 1993, Vol. 21, No. 25 5843-5845
© 1993

MOLECULAR BIOLOGY

Differences in the accessibility of methylated and unmethylated DNA to DNase I

Stefan Kochanek+, Doris Renz and Walter Doerfler*

Institute of Genetics, University of Cologne Weyertal 121, D-50931 Cologne, Germany

To whom correspondence should be addressed

Received September 30, 1993. Revised November 16, 1993. Accepted November 16, 1993.

DNase I binds in the minor groove of DNA and is used as an enzymatic tool to investigate the interaction of proteins with DNA. Here we show that the major groove located 5-methyldeoxycytidine can enhance or inhibit the cleavage rates of DNA by DNase I. This effect may be caused in part by changes in DNA structure affecting the accessibility of the minor groove of DNA to DNase I.

+Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030, USA


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