Nucleic Acids Research, 1993, Vol. 21, No. 25 5890-5895
© 1993
MOLECULAR BIOLOGY |
Deficient repair of the transcribed strand of active genes in Cockayne's syndrome cells
1MGC – Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Wassenaarseweg 72, 2333 AL Leiden 2J.A.Cohen Institute, Interuniversity Research Institute for Radiation Protection and Radiopathology Leiden, The Netherlands 3The Trafford Centre for Medical Research, University of Sussex, Falmer Brighton BN1 9RY, UK
*Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Wassenaarseweg, 72, 2333 AL Leiden, The Netherlands
Received September 20, 1993. Revised November 22, 1993. Accepted November 22, 1993.
Removal of ultraviolet light induced cyclobutane pyrimidine dimers (CPD) from active and inactive genes was analyzed in cells derived from patients suffering from the hereditary disease Cockayne's syndrome (CS) using strand specific probes. The results indicate that the defect in CS cells affects two levels of repair of lesions in active genes. Firstly, CS cells are deficient in selective repair of the transcribed strand of active genes. In these cells the rate and efficiency of repair of CPD are equal for the transcribed and the nontranscribed strand of the active ADA and DHFR genes. In normal cells on the other hand, the transcribed strand of these genes is repaired faster than the nontranscribed strand. However, the nontranscribed strand is still repaired more efficiently than the inactive 754 gene and the gene coding for coagulation factor IX. Secondly, the repair level of active genes in CS cells exceeds that of inactive loci but is slower than the nontranscribed strand of active genes in normal cells. Our results support the model that CS cells lack a factor which is involved in targeting repair enzymes specifically towards DNA damage located in (potentially) active DNA.
+Department of Biochemistry and Molecular Biology, Vrije Universiteit, de Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
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|
|
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|
|
|
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|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
W. L. de Laat, N. G.J. Jaspers, and J. H.J. Hoeijmakers Molecular mechanism of nucleotide excision repair Genes & Dev., April 1, 1999; 13(7): 768 - 785. [Full Text]
|
|
|
|
|
|
M. Tijsterman and J. Brouwer Rad26, the Yeast Homolog of the Cockayne Syndrome B Gene Product, Counteracts Inhibition of DNA Repair Due to RNA Polymerase II Transcription J. Biol. Chem., January 15, 1999; 274(3): 1199 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Francis and A. J. Rainbow UV-enhanced reactivation of a UV-damaged reporter gene suggests transcription-coupled repair is UV-inducible in human cells Carcinogenesis, January 1, 1999; 20(1): 19 - 26. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
M. F. van Oosterwijk, R. Filon, A. J. L. de Groot, A. A. van Zeeland, and L. H. F. Mullenders Lack of Transcription-coupled Repair of Acetylaminofluorene DNA Adducts in Human Fibroblasts Contrasts Their Efficient Inhibition of Transcription J. Biol. Chem., May 29, 1998; 273(22): 13599 - 13604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Citterio, S. Rademakers, G. T. J. van der Horst, A. J. van Gool, J. H.J. Hoeijmakers, and W. Vermeulen Biochemical and Biological Characterization of Wild-type and ATPase-deficient Cockayne Syndrome B Repair Protein J. Biol. Chem., May 8, 1998; 273(19): 11844 - 11851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tu, S. Bates, and G. P. Pfeifer Sequence-specific and Domain-specific DNA Repair in Xeroderma Pigmentosum and Cockayne Syndrome Cells J. Biol. Chem., August 15, 1997; 272(33): 20747 - 20755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tijsterman, R. A. Verhage, P. van de Putte, J. G. T.-d. Jong, and J. Brouwer Transitions in the coupling of transcription and nucleotide excision repair within RNA polymerase II-transcribed genes of Saccharomyces cerevisiae PNAS, July 22, 1997; 94(15): 8027 - 8032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nouspikel, P. Lalle, S. A. Leadon, P. K. Cooper, and S. G. Clarkson A common mutational pattern in Cockayne syndrome patients from xeroderma pigmentosum group G: Implications for a second XPG function PNAS, April 1, 1997; 94(7): 3116 - 3121. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Otrin, M McLenigan, M Takao, A. Levine, and M Protic Translocation of a UV-damaged DNA binding protein into a tight association with chromatin after treatment of mammalian cells with UV light J. Cell Sci., January 5, 1997; 110(10): 1159 - 1168. [Abstract] [PDF]
|
|
|
|
|
|
S. N. Guzder, Y. Habraken, P. Sung, L. Prakash, and S. Prakash RAD26, the Yeast Homolog of Human Cockayne's Syndrome Group B Gene, Encodes a DNA-dependent ATPase J. Biol. Chem., August 2, 1996; 271(31): 18314 - 18317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. O. Murad, J. de Cock, D. Brown, and M. J. Smerdon Variations in Transcription-Repair Coupling in Mouse Cells J. Biol. Chem., February 24, 1995; 270(8): 3949 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Sancar Mechanisms of DNA excision repair Science, December 23, 1994; 266(5193): 1954 - 1956. [PDF]
|
|
|
|
|
|
Y. Nakatsu, H. Asahina, E. Citterio, S. Rademakers, W. Vermeulen, S. Kamiuchi, J.-P. Yeo, M.-C. Khaw, M. Saijo, N. Kodo, et al. XAB2, a Novel Tetratricopeptide Repeat Protein Involved in Transcription-coupled DNA Repair and Transcription J. Biol. Chem., November 3, 2000; 275(45): 34931 - 34937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
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