Nucleic Acids Research, 1993, Vol. 21, No. 25 5940-5944
© 1993
MOLECULAR BIOLOGY |
The fission yeast rad22 gene, having a function in matingtype switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae
Institut für Genetik, Biozentrum, Technische Universität Braunschweig Spielmannstraße 7, D-38106 Braunschweig, Germany
To whom correspondence should be addressed
Received August 31, 1993. Revised November 16, 1993. Accepted November 16, 1993.
The gene rad22 of the fission yeast Schizosaccharomycespombe has a function in DNA repair and matingtype switching. We have cloned the rad22 gene from a genomic gene bank by functional complementation of the switching defect. An open reading frame coding for a putative protein of 469 amino acids was found by sequence analyses. The rad22 gene contains no intron. A region of 126 amino acids in the N-terminal half of the Rad22 protein has significant homologies (56% identity and 36% similarity) to the Rad52 protein of Saccharomyces cerevisiae. A rad22 disruption strain was constructed which seems to be inviable in a homothallic background. Southern blot analyses have shown that the rad22-67 mutant frequently gives rise to deletions in the mating-type region. These data indicate that the Rad22 protein has a function in the repair of DNA double-strand breaks.
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