Nucleic Acids Research, 1993, Vol. 21, No. 25 5945-5949
© 1993
MOLECULAR BIOLOGY |
Identification of a chicken RAD52 homologue suggests conservation of the RAD52 recombination pathway throughout the evolution of higher eukaryotes
Basel Institut of Immunology GrenzacherstraBe 487, CH-4005 Basel, Switzerland 1lnstitut of Genetics, TU Braunschweig SpielmannstraGe 7, D-38106 Braunschweig, Germany 2Institute of General Microbiology, University of Bern Baltzer-StraBe 4, CH-3012 Bern, Switzerland
Received August 27, 1993. Revised November 16, 1993. Accepted November 16, 1993.
Degenerate oligonucleotides encoding conserved regions of the Rad52 protein of S.cerevisiae and its homologue, the Rad22 protein of S.pombe, were used to clone a chicken RAD52 counterpart by the polymerase chain reaction. Sequence comparison of the chicken and yeast proteins reveals a strongly conserved region between positions 40 and 178 of the chicken Rad52 sequence indicating that this part of the protein is under strong evolutionary pressure. The first 39 amino acids and the 3' end of the chicken Rad52 homologue does not share significant similarity with the yeast proteins. High abundance of the mRNA in testis makes it likely that the chicken Rad52 protein plays a role in meiotic recombination.
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