Nucleic Acids Research, 1993, Vol. 21, No. 3 381-386
© 1993
MOLECULAR BIOLOGY |
Reversion of a transcriptionally defective MHC class II-negative human B-cell mutant
The International Institute of Genetics and Biophysics, CNR, via Guglielmo Marconi 10, I-80125 and The National Cancer Institute ‘Fondazione Pascale’, Naples, Italy
*To whom correspondence should be addressed
Received November 26, 1992. Accepted January 11, 1993.
RJ2.2.5, a mutant derived from the human B-lymphoma cell, Raji, is unable to express the MHC class II genes because of a recessive transcriptional defect attributed to the lack of an activator function. We report the isolation of a RJ2.2.5 revertant, namely AR, in which the expression of the mRNAs encoded by these genes Is restored. Comparison of the binding of nuclear extracts or of partially purified nuclear preparations from the wild-type, the mutant and the revertant cells to a conserved MHC class II promoter element, the X-box, showed no alteration in the mobility of the complexes thus formed. However, in extracts from RJ2.2.5, and other MHC class II negative cell lines, such as HeLa, the amount of compiex observed was signlficantiy higher than in wild-type Raji cells. Furthermore, the binding activity exhibited by the AR revertant was lower than that of the RJ2.2.5 and higher than that of Raji. The use of specific monoclonal antibodies indicated that in all cases c-Jun and c-Fos or antlgenically related proteins were required for binding. An inverse correlation between the level of DNA-protein complex formed and the level of MHC class II gene mRNA expressed in the three cell lines was apparent, suggesting that overexpresslon of a DNA binding factor forming complexes with class ii promoter elements may cause repression of MHC class II transcription. A model which reconciles the previously ascertained recessivity of the phenotype of the mutation carried by RJ2.2.5 with the findings reported here is discussed.